caa a22 4500 475745507 CHVBK 20180406123512.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s002849910009 doi (NATIONALLICENCE)springer-10.1007/s002849910009 The Basal-Level Expression of the Cytochrome P450BM-1 Gene Is Negatively Affected by the bm1P1 Gene of Bacillus megaterium [Elektronische Daten] [Gwo-Chyuan Shaw, Yi-Huang Hsueh, Hsun-Sheng Kao] Abstract.: Evidence against the Bm1P1 protein as a positive transcription factor for barbiturate-mediated induction of cytochrome P450BM-1 in Bacillus megaterium has been provided in our previous report. In the present study, we show that the basal-level expression of the P450BM-1 promoter-xylE transcriptional fusion is significantly reduced when the bm1P1 gene is present on the same plasmid. Moreover, disruption of the chromosomal bm1P1 gene results in enhanced expression of the P450BM-1 promoter-xylE fusion on a multicopy plasmid. By using a binary vector system, we found that expression of the P450BM-1 promoter-xylE fusion from a high-copy plasmid is substantially repressed by expression of bm1P1 from a low-copy plasmid. Taken together, these results suggest that the basal-level expression of P450BM-1 is negatively affected by bm1P1. Possible mechanisms for this control are discussed. Springer-Verlag New York Inc., 2000 Shaw Gwo-Chyuan Institute of Biochemistry, School of Life Science, National Yang-Ming University, Taipei, Taiwan, Republic of China, TW aut Hsueh Yi-Huang Institute of Biochemistry, School of Life Science, National Yang-Ming University, Taipei, Taiwan, Republic of China, TW aut Kao Hsun-Sheng Institute of Biochemistry, School of Life Science, National Yang-Ming University, Taipei, Taiwan, Republic of China, TW aut https://doi.org/10.1007/s002849910009 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910009 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shaw Gwo-Chyuan Institute of Biochemistry, School of Life Science, National Yang-Ming University, Taipei, Taiwan, Republic of China, TW aut NATIONALLICENCE 700 1- Hsueh Yi-Huang Institute of Biochemistry, School of Life Science, National Yang-Ming University, Taipei, Taiwan, Republic of China, TW aut NATIONALLICENCE 700 1- Kao Hsun-Sheng Institute of Biochemistry, School of Life Science, National Yang-Ming University, Taipei, Taiwan, Republic of China, TW aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer