caa a22 4500 475745523 CHVBK 20180406123512.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s002849910001 doi (NATIONALLICENCE)springer-10.1007/s002849910001 Potential Utility of Random Amplified Polymorphic DNA (RAPD) and Restriction Endonuclease Assay (REA) as Typing Systems for Madurella mycetomatis [Elektronische Daten] [Maria M. Lopes, Graciete Freitas, Patrick Boiron] Abstract.: Two molecular methods were compared, random amplified polymorphic DNA (RAPD) and restriction endonuclease analysis (REA), in order to evaluate their ability to discriminate, and to characterize Madurella mycetomatis strains isolated from human mycetomas in different parts of the world. Both methods were able to cluster the Madurella mycetomatis isolates into the same number of distinct typing groups. However, RAPD, presenting several advantages over REA such as its rapidity, simplicity, and the accessibility for implementation in the laboratory, is a more sensitive and reproducible tool for the study of Madurella mycetomatis epidemiology than REA. Springer-Verlag New York Inc., 2000 Lopes Maria M. Faculdade de Farmácia, Universidade de Lisboa, Departamento de Microbiologia, Av. das Forças Armadas, 1649-019 Lisboa, Portugal, PT aut Freitas Graciete Faculdade de Farmácia, Universidade de Lisboa, Departamento de Microbiologia, Av. das Forças Armadas, 1649-019 Lisboa, Portugal, PT aut Boiron Patrick Institut Pasteur, Unité de Mycologie, 25-28, Rue du Docteur Roux, 75724 Paris Cedex 15, France, FR aut https://doi.org/10.1007/s002849910001 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910001 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lopes Maria M. Faculdade de Farmácia, Universidade de Lisboa, Departamento de Microbiologia, Av. das Forças Armadas, 1649-019 Lisboa, Portugal, PT aut NATIONALLICENCE 700 1- Freitas Graciete Faculdade de Farmácia, Universidade de Lisboa, Departamento de Microbiologia, Av. das Forças Armadas, 1649-019 Lisboa, Portugal, PT aut NATIONALLICENCE 700 1- Boiron Patrick Institut Pasteur, Unité de Mycologie, 25-28, Rue du Docteur Roux, 75724 Paris Cedex 15, France, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer