caa a22 4500 475745566 CHVBK 20180406123512.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s002849910010 doi (NATIONALLICENCE)springer-10.1007/s002849910010 Genome Stability of Bacillus thuringiensis subsp. israelensis Isolates [Elektronische Daten] [Jonas Ankarloo, Dominique A. Caugant, Bjarne M. Hansen, Alexandra Berg, Anne-Brit Kolstø, Ann Lövgren] Abstract.: Swedish soil isolates biochemically classified as Bacillus thuringiensis subsp. israelensis were further examined for genetic diversity by multilocus enzyme electrophoresis (MLEE), random amplified polymorphic DNA analysis (RAPD), pulse field gel electrophoresis (PFGE), and Southern blotting, and were compared with reference strains. All the tested strains belonging to the Bt. israelensis serotype H14 were found to be identical, as judged from the RAPD analysis. MLEE analysis gave a similar result; only one H14 strain was found to differ from the remaining H14 strains by one null allele. PFGE analysis confirmed a very close relationship between the H14 strains but revealed an SfiI restriction fragment of variable size. Southern blot analyses were carried out with probes for the chromosomally encoded flagellin gene(s) and the plasmid-encoded mosquitocidal toxins. All probes gave similar hybridization patterns in the H14 strains. The mosquito toxin probes hybridized only to the H14 strains, except for one probe hybridizing to strain 6:3, which was originally isolated from the same soil sample as strains 6:11 and 6:12. Because the RAPD, MLEE, and PFGE analyses showed that strain 6:3 appears to be unrelated to strains 6:11 and 6:12, the presence of a mosquito toxin sequence in strain 6:3 may suggest that gene transfer has occurred. Springer-Verlag New York Inc., 2000 Ankarloo Jonas Department of Microbiology, Stockholm University, S-106 91 Stockholm, Sweden, SE aut Caugant Dominique A. Department of Bacteriology, National Institute of Public Health, P.O. Box 4404 Torshov, N-0403 Oslo, Norway, NO aut Hansen Bjarne M. Department of Marine Ecology and Microbiology, National Environmental Research Institute, Frederiksborgvej 399, P.O. Box 358, DK-4000 Roskilde, Denmark, DK aut Berg Alexandra Department of Microbiology, Stockholm University, S-106 91 Stockholm, Sweden, SE aut Kolstø Anne-Brit Department of Microbiology, Biotechnology Center of Oslo, University of Oslo, P.O. Box 1125 Blindern, N-0316 Oslo, Norway, NO aut Lövgren Ann Department of Microbiology, Stockholm University, S-106 91 Stockholm, Sweden, SE aut https://doi.org/10.1007/s002849910010 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910010 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ankarloo Jonas Department of Microbiology, Stockholm University, S-106 91 Stockholm, Sweden, SE aut NATIONALLICENCE 700 1- Caugant Dominique A. Department of Bacteriology, National Institute of Public Health, P.O. Box 4404 Torshov, N-0403 Oslo, Norway, NO aut NATIONALLICENCE 700 1- Hansen Bjarne M. Department of Marine Ecology and Microbiology, National Environmental Research Institute, Frederiksborgvej 399, P.O. Box 358, DK-4000 Roskilde, Denmark, DK aut NATIONALLICENCE 700 1- Berg Alexandra Department of Microbiology, Stockholm University, S-106 91 Stockholm, Sweden, SE aut NATIONALLICENCE 700 1- Kolstø Anne-Brit Department of Microbiology, Biotechnology Center of Oslo, University of Oslo, P.O. Box 1125 Blindern, N-0316 Oslo, Norway, NO aut NATIONALLICENCE 700 1- Lövgren Ann Department of Microbiology, Stockholm University, S-106 91 Stockholm, Sweden, SE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer