caa a22 4500 475745582 CHVBK 20180406123512.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s002849910013 doi (NATIONALLICENCE)springer-10.1007/s002849910013 Cloning and Characterization of the Gene Encoding O -Acetylserine Lyase from Streptococcus suis [Elektronische Daten] [Makoto Osaki, Daisuke Takamatsu, Naotoshi Tsuji, Tsutomu Sekizaki] Abstract.: We have cloned and sequenced a gene encoding O-acetylserine lyase from Streptococcus suis. The gene encodes a protein of 309 amino acids with a calculated molecular mass of 32,038 Da. The deduced amino acid sequence showed more extensive similarities to the CysK proteins than to the CysM proteins of other bacteria. The cloned gene was inserted into a pTrcHisB histidine hexamer expression vector. A 38-kDa fusion protein was expressed in a cysMK auxotrophic mutant of Salmonella typhimurium and complemented the auxotrophic properties of the mutant. Furthermore, the transformants could grow in minimal defined media supplemented with not only sulfide but also thiosulfate as a sole sulfur source. These data indicated that the cloned gene encodes a protein that was a functional homolog of the CysM in S. typhimurium. Springer-Verlag New York Inc., 2000 Osaki Makoto National Institute of Animal Health, Kannondai 3-1-1, Tsukuba, Ibaraki 305-0856, Japan, JP aut Takamatsu Daisuke National Institute of Animal Health, Kannondai 3-1-1, Tsukuba, Ibaraki 305-0856, Japan, JP aut Tsuji Naotoshi National Institute of Animal Health, Kannondai 3-1-1, Tsukuba, Ibaraki 305-0856, Japan, JP aut Sekizaki Tsutomu National Institute of Animal Health, Kannondai 3-1-1, Tsukuba, Ibaraki 305-0856, Japan, JP aut https://doi.org/10.1007/s002849910013 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910013 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Osaki Makoto National Institute of Animal Health, Kannondai 3-1-1, Tsukuba, Ibaraki 305-0856, Japan, JP aut NATIONALLICENCE 700 1- Takamatsu Daisuke National Institute of Animal Health, Kannondai 3-1-1, Tsukuba, Ibaraki 305-0856, Japan, JP aut NATIONALLICENCE 700 1- Tsuji Naotoshi National Institute of Animal Health, Kannondai 3-1-1, Tsukuba, Ibaraki 305-0856, Japan, JP aut NATIONALLICENCE 700 1- Sekizaki Tsutomu National Institute of Animal Health, Kannondai 3-1-1, Tsukuba, Ibaraki 305-0856, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer