caa a22 4500 475745590 CHVBK 20180406123512.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s002849910008 doi (NATIONALLICENCE)springer-10.1007/s002849910008 Molecular Identification of Potentially Probiotic Lactobacilli [Elektronische Daten] [Denis Roy, Pierre Ward, Daniel Vincent, Francine Mondou] Abstract.: The rRNA-targeted oligonucleotide probes are useful for the identification of Lactobacillus acidophilus, L. gasseri, L. johnsonii, L. crispatus, and L. amylovorus. However, the oligonucleotide probe designed for L. helveticus hybridized with nucleic acids of type strains of L. gallinarum and L. helveticus. Hence, the similarity among the 73 strains of lactobacilli was evaluated on the basis of their randomly amplified polymorphic DNA (RAPD) profiles derived from five single-primer reactions. These strains were grouped into seven clusters at a similarity level of 30%, which corresponded to six separate species of the L. acidophilus complex (L. johnsonii, L. gallinarum, L. amylovorus, L. crispatus, L. acidophilus, and L. gasseri, respectively) and L. helveticus. For the first time, strains of L. gallinarum were characterized by RAPD and PFGE analyses. The genome length in that species was estimated to be near 1.45 Mb with the summation of ApaI fragments, and near 1.95 Mb with the summation of SmaI fragments. Springer-Verlag New York Inc., 2000 Roy Denis Food Research and Development Centre, Agriculture and Agrifood Canada, 3600 Casavant Blvd. West, Saint-Hyacinthe, Québec, Canada, J2S 8E3, CA aut Ward Pierre Food Research and Development Centre, Agriculture and Agrifood Canada, 3600 Casavant Blvd. West, Saint-Hyacinthe, Québec, Canada, J2S 8E3, CA aut Vincent Daniel Food Research and Development Centre, Agriculture and Agrifood Canada, 3600 Casavant Blvd. West, Saint-Hyacinthe, Québec, Canada, J2S 8E3, CA aut Mondou Francine Rosell Institute Inc., Montréal, Québec, Canada, CA aut https://doi.org/10.1007/s002849910008 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910008 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Roy Denis Food Research and Development Centre, Agriculture and Agrifood Canada, 3600 Casavant Blvd. West, Saint-Hyacinthe, Québec, Canada, J2S 8E3, CA aut NATIONALLICENCE 700 1- Ward Pierre Food Research and Development Centre, Agriculture and Agrifood Canada, 3600 Casavant Blvd. West, Saint-Hyacinthe, Québec, Canada, J2S 8E3, CA aut NATIONALLICENCE 700 1- Vincent Daniel Food Research and Development Centre, Agriculture and Agrifood Canada, 3600 Casavant Blvd. West, Saint-Hyacinthe, Québec, Canada, J2S 8E3, CA aut NATIONALLICENCE 700 1- Mondou Francine Rosell Institute Inc., Montréal, Québec, Canada, CA aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer