caa a22 4500 475745612 CHVBK 20180406123512.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s002849910006 doi (NATIONALLICENCE)springer-10.1007/s002849910006 Use of 16S rDNA Sequences as Signature Characters to Identify Xylella fastidiosa [Elektronische Daten] [Jianchi Chen, Donna Banks, Robert L. Jarret, Chung J. Chang, Barbara J. Smith] Abstract.: The nucleotide sequences of 16S rDNAs (coding for the small subunit ribosomal RNAs) were used to identify Xylella fastidiosa, a nutritionally fastidious plant pathogenic bacterium. The near-complete 16S rDNAs from nine strains of Xyl. fastidiosa, including seven pathotypes and one strain of Xanthomonas campestris pv. campestris, were amplified through PCR with two conserved primers (forward primer 5′-AGA GTT TGA TCC TGG CTC AG-3′ and reverse primer 5′-AAG GAG GTG ATC CAG CC-3′) and sequenced. The 16S sequences were compared with all eukaryote and prokaryote DNA entries in GenBank database. A Xyl. fastidiosa 16S rDNA sequence, M26601, was determined to be the most similar to all the near-complete (1537 bp) and partial 5′ end sequences from Xyl. fastidiosa, but not those from the Xanthomonas strain. A 20-bp oligonucleotide (5′-TTG GTA GTA ATA CCA TGG GT-3′) was found to be highly characteristic of Xyl. fastidiosa. Since the 16S rDNA of Xyl. fastidiosa strains are highly homologous and characteristically different from other bacteria, including the most closely related Xanthomonas, 16S rDNA sequences can be used as signature characters to identify this bacterium. Springer-Verlag New York Inc., 2000 Chen Jianchi Center for Viticultural Science, Florida A&M University, Tallahassee, FL 32307, USA, US aut Banks Donna Center for Viticultural Science, Florida A&M University, Tallahassee, FL 32307, USA, US aut Jarret Robert L. USDA-ARS, Department of Plant Genetic Resources, Griffin, GA 30223, USA, US aut Chang Chung J. Department of Plant Pathology, University of Georgia, 1109 Experiment St., Griffin, GA 30223, USA, US aut Smith Barbara J. USDA-ARS, Small Fruit Research Station, 306 S. High St., Poplarville, MS 39470, USA, US aut https://doi.org/10.1007/s002849910006 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910006 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Jianchi Center for Viticultural Science, Florida A&M University, Tallahassee, FL 32307, USA, US aut NATIONALLICENCE 700 1- Banks Donna Center for Viticultural Science, Florida A&M University, Tallahassee, FL 32307, USA, US aut NATIONALLICENCE 700 1- Jarret Robert L. USDA-ARS, Department of Plant Genetic Resources, Griffin, GA 30223, USA, US aut NATIONALLICENCE 700 1- Chang Chung J. Department of Plant Pathology, University of Georgia, 1109 Experiment St., Griffin, GA 30223, USA, US aut NATIONALLICENCE 700 1- Smith Barbara J. USDA-ARS, Small Fruit Research Station, 306 S. High St., Poplarville, MS 39470, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer