caa a22 4500 475745620 CHVBK 20180406123512.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s002840010069 doi (NATIONALLICENCE)springer-10.1007/s002840010069 Genomic DNA Fingerprinting of Oenococcus oeni Strains by Pulsed-Field Gel Electrophoresis and Randomly Amplified Polymorphic DNA-PCR [Elektronische Daten] [Giacomo Zapparoli, Cristina Reguant, Albert Bordons, Sandra Torriani, Franco Dellaglio] Abstract.: Genetic diversity of 60 Oenococcus oeni strains from different wines was evaluated by numerical analysis of (i) pulsed-field gel electrophoresis (PFGE) patterns with endonuclease ApaI and (ii) randomly amplified polymorphic DNA (RAPD)-PCR fingerprints with four oligonucleotide primers. Sixty-two percent of the strains could be distinguished by PFGE, whereas most strains were identified by distinct RAPD-PCR profiles and associated according to the geographical origin. Because of its rapidity and reliability, RAPD-PCR appeared to be a suitable method for typing and monitoring O. oeni strains in winemaking. Springer-Verlag New York Inc., 2000 Zapparoli Giacomo Dipartimento Scientifico e Tecnologico, Università degli Studi di Verona, strada Le Grazie, 37134 Verona, Italy, IT aut Reguant Cristina Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, Pl. Imperial Tarraco, 1, 43005 Tarragona, Spain, ES aut Bordons Albert Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, Pl. Imperial Tarraco, 1, 43005 Tarragona, Spain, ES aut Torriani Sandra Istituto di Industrie Agrarie, Università degli Studi di Catania, via Santa Sofia 98, 95124 Catania, Italy, IT aut Dellaglio Franco Dipartimento Scientifico e Tecnologico, Università degli Studi di Verona, strada Le Grazie, 37134 Verona, Italy, IT aut https://doi.org/10.1007/s002840010069 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010069 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zapparoli Giacomo Dipartimento Scientifico e Tecnologico, Università degli Studi di Verona, strada Le Grazie, 37134 Verona, Italy, IT aut NATIONALLICENCE 700 1- Reguant Cristina Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, Pl. Imperial Tarraco, 1, 43005 Tarragona, Spain, ES aut NATIONALLICENCE 700 1- Bordons Albert Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, Pl. Imperial Tarraco, 1, 43005 Tarragona, Spain, ES aut NATIONALLICENCE 700 1- Torriani Sandra Istituto di Industrie Agrarie, Università degli Studi di Catania, via Santa Sofia 98, 95124 Catania, Italy, IT aut NATIONALLICENCE 700 1- Dellaglio Franco Dipartimento Scientifico e Tecnologico, Università degli Studi di Verona, strada Le Grazie, 37134 Verona, Italy, IT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer