caa a22 4500 47574568X CHVBK 20180406123512.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s002840010071 doi (NATIONALLICENCE)springer-10.1007/s002840010071 Isolation of a Gene from Burkholderia cepacia IS-16 Encoding a Protein That Facilitates Phosphatase Activity [Elektronische Daten] [Hilda Rodríguez, Gian M. Rossolini, Tania Gonzalez, Jiping Li, Bernard R. Glick] Abstract.: A genomic library from Burkholderia cepacia IS-16 was constructed in Escherichia coli by partial Sau3AI digestion of the chromosomal DNA, with the plasmid vector Bluescript SK. This library was screened for clones able to grow as green stained colonies on selective medium developed for detecting phosphatase-positive colonies. Three green-stained clones (pFS1, pFS2, and pFS3) carried recombinant plasmids harboring DNA inserts of 5.0, 8.0, and 0.9 kb, respectively. DNA hybridization experiments demonstrated the presence of overlapping DNA fragments in the three clones and that these three clones were all derived from Burkholderia cepacia IS-16 genomic DNA. DNA sequence analysis, together with polyacrylamide gels of proteins encoded by E. coli containing pFS3, suggested that the isolated 0.9-kb DNA fragment encodes the functional portion of a phosphate transport protein. Springer-Verlag New York Inc., 2000 Rodríguez Hilda Department of Microbiology, Cuban Research Institute on Sugarcane By-Products, Havana, Cuba, CU aut Rossolini Gian M. Department of Molecular Biology, University of Siena, Italy, IT aut Gonzalez Tania Department of Microbiology, Cuban Research Institute on Sugarcane By-Products, Havana, Cuba, CU aut Li Jiping Department of Biology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1, CA aut Glick Bernard R. Department of Biology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1, CA aut https://doi.org/10.1007/s002840010071 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010071 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rodríguez Hilda Department of Microbiology, Cuban Research Institute on Sugarcane By-Products, Havana, Cuba, CU aut NATIONALLICENCE 700 1- Rossolini Gian M. Department of Molecular Biology, University of Siena, Italy, IT aut NATIONALLICENCE 700 1- Gonzalez Tania Department of Microbiology, Cuban Research Institute on Sugarcane By-Products, Havana, Cuba, CU aut NATIONALLICENCE 700 1- Li Jiping Department of Biology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1, CA aut NATIONALLICENCE 700 1- Glick Bernard R. Department of Biology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1, CA aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer