caa a22 4500 475745698 CHVBK 20180406123512.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s002840010080 doi (NATIONALLICENCE)springer-10.1007/s002840010080 Recovery of Bacillus thuringiensis from Marine Sediments of Japan [Elektronische Daten] [Minoru Maeda, Eiichi Mizuki, Yukinobu Nakamura, Toshitake Hatano, Michio Ohba] Abstract.: Marine sediments from a Japanese bay were examined for the occurrence of Bacillus thuringiensis. Of 1313 colonies belonging to the Bacillus cereus/B. thuringiensis group, 22 (1.7%) were allocated to B. thuringiensis. Marine isolates of B. thuringiensis consisted of heterogeneous multiple H serogroups; 10 isolates were assigned to the eight serovars (kurstaki, sumiyoshiensis, sotto, aizawai, darmstadiensis, thompsoni, neoleonensis, and higo); two motile isolates failed to react with the reference antisera; and the others were serologically untestable. Insecticidal activities were associated with two kurstaki isolates (toxic to both Lepidoptera and Diptera) and a higo isolate (Diptera-specific). None of the parasporal inclusion proteins of the 22 isolates exhibited in vitro cytotoxic activity against two vertebrate cells, sheep erythrocytes and HeLa cells. All B. thuringiensis isolates had no halophilism, although seawater-based medium supported their growth, sporulation, and formation of parasporal inclusions. Springer-Verlag New York Inc., 2000 Maeda Minoru Kyushu Medical Co., Ltd., 13-4 Ohte-machi, Kokurakita-ku, Kitakyushu, Fukuoka 803-0814, Japan, JP aut Mizuki Eiichi Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa, Kurume, Fukuoka 839-0816, Japan, JP aut Nakamura Yukinobu Kyushu Medical Co., Ltd., 13-4 Ohte-machi, Kokurakita-ku, Kitakyushu, Fukuoka 803-0814, Japan, JP aut Hatano Toshitake Kyushu Medical Co., Ltd., 13-4 Ohte-machi, Kokurakita-ku, Kitakyushu, Fukuoka 803-0814, Japan, JP aut Ohba Michio Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka 812-8581, Japan, JP aut https://doi.org/10.1007/s002840010080 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010080 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Maeda Minoru Kyushu Medical Co., Ltd., 13-4 Ohte-machi, Kokurakita-ku, Kitakyushu, Fukuoka 803-0814, Japan, JP aut NATIONALLICENCE 700 1- Mizuki Eiichi Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa, Kurume, Fukuoka 839-0816, Japan, JP aut NATIONALLICENCE 700 1- Nakamura Yukinobu Kyushu Medical Co., Ltd., 13-4 Ohte-machi, Kokurakita-ku, Kitakyushu, Fukuoka 803-0814, Japan, JP aut NATIONALLICENCE 700 1- Hatano Toshitake Kyushu Medical Co., Ltd., 13-4 Ohte-machi, Kokurakita-ku, Kitakyushu, Fukuoka 803-0814, Japan, JP aut NATIONALLICENCE 700 1- Ohba Michio Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka 812-8581, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer