caa a22 4500 47574571X CHVBK 20180406123512.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s002840010072 doi (NATIONALLICENCE)springer-10.1007/s002840010072 Transformation of Internal Extracellular Bacteria Isolated from Rhagoletis completa Cresson Gut with Enhanced Green Fluorescent Protein [Elektronische Daten] [John J. Peloquin, Lyudmila Kuzina, Carol R. Lauzon, Thomas A. Miller] Abstract.: We discovered Zeocin™ is an effective antibiotic against Enterobacter agglomerans and Klebsiella pneumoniae strains isolated from the walnut husk fly (Rhagoletis completa Cresson: Family Tephritidae) and that bleomycin resistance can be used as a selective marker in transforming plasmids. We transformed Ent. agglomerans and K. pneumoniae strains originally isolated from their close association with R. completa gut to produce enhanced green fluorescent protein, a variant of green fluorescent protein in the first demonstration of genetic transformation of internal extracellular bacteria isolated from a tephritid pest. We report methods for plasmid-mediated transformation of these bacteria, the expression of fluorescent marker protein from the transforming plasmids, and the stability of the transforming plasmid in the bacteria. We also discuss applications of this technology in the study of pest biology and control implementation. Springer-Verlag New York Inc., 2000 Peloquin John J. Department of Entomology, University of California, Riverside, Riverside, CA 92521, USA, US aut Kuzina Lyudmila Department of Entomology, University of California, Riverside, Riverside, CA 92521, USA, US aut Lauzon Carol R. Department of Biological Sciences, California State University-Hayward, Hayward, CA 94542, USA, US aut Miller Thomas A. Department of Entomology, University of California, Riverside, Riverside, CA 92521, USA, US aut https://doi.org/10.1007/s002840010072 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010072 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Peloquin John J. Department of Entomology, University of California, Riverside, Riverside, CA 92521, USA, US aut NATIONALLICENCE 700 1- Kuzina Lyudmila Department of Entomology, University of California, Riverside, Riverside, CA 92521, USA, US aut NATIONALLICENCE 700 1- Lauzon Carol R. Department of Biological Sciences, California State University-Hayward, Hayward, CA 94542, USA, US aut NATIONALLICENCE 700 1- Miller Thomas A. Department of Entomology, University of California, Riverside, Riverside, CA 92521, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer