caa a22 4500 475745728 CHVBK 20180406123512.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s002840010079 doi (NATIONALLICENCE)springer-10.1007/s002840010079 Survival of Salmonella Species in River Water [Elektronische Daten] [Jorge W. Santo Domingo, Stephen Harmon, Jason Bennett] Abstract.: The survival of four Salmonella strains in river water microcosms was monitored by culturing techniques, direct counts, whole-cell hybridization, scanning electron microscopy, and resuscitation techniques via the direct viable count method and flow cytometry. Plate counts of bacteria resuspended in filtered and untreated river water decreased several orders of magnitude within the first week of incubation, while they did not decrease as rapidly in autoclaved water. In situ hybridization studies suggested a rapid decrease in ribosomal content, as determined by the drastic decrease in the number of detectable cells after 72 h. In contrast, direct counts remained relatively constant during 45 days in all microcosoms. Although the culturable counts of two bacterial strains in filtered water after 31 days represented approximately 0.001% of the total counts, direct viable counts and resuscitation studies with a dilution series suggested that the number of viable bacteria was at least four orders of magnitude higher. Additionally, notable changes in forward scatter and in nucleic acid content were observed only after 4 h of nutrient amendments by flow cytometry. However, cells from the resuscitation experiments did not grow on solid media unless cell-free supernatant from viable cultures was added during the resuscitation period. The results in this study suggest the presence of a not immediately culturable status in Salmonella. Springer-Verlag New York Inc., 2000 Santo Domingo Jorge W. U.S. Environmental Protection Agency, National Exposure Research Laboratory, 26 W. Martin Luther King Dr., Cincinnati, OH 45268 USA, US aut Harmon Stephen National Risk Management Research Laboratory, 26 W. Martin Luther King Dr., Cincinnati, OH 45268, USA, US aut Bennett Jason U.S. Environmental Protection Agency, National Exposure Research Laboratory, 26 W. Martin Luther King Dr., Cincinnati, OH 45268 USA, US aut https://doi.org/10.1007/s002840010079 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010079 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Santo Domingo Jorge W. U.S. Environmental Protection Agency, National Exposure Research Laboratory, 26 W. Martin Luther King Dr., Cincinnati, OH 45268 USA, US aut NATIONALLICENCE 700 1- Harmon Stephen National Risk Management Research Laboratory, 26 W. Martin Luther King Dr., Cincinnati, OH 45268, USA, US aut NATIONALLICENCE 700 1- Bennett Jason U.S. Environmental Protection Agency, National Exposure Research Laboratory, 26 W. Martin Luther King Dr., Cincinnati, OH 45268 USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer