caa a22 4500 475745736 CHVBK 20180406123512.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s002840010070 doi (NATIONALLICENCE)springer-10.1007/s002840010070 Diversity of Cyanobacterial Hydrogenases, a Molecular Approach [Elektronische Daten] [Paula Tamagnini, José-Luís Costa, Lígia Almeida, Maria-José Oliveira, Roberto Salema, Peter Lindblad] Abstract.: In an effort to elucidate the diversity of cyanobacterial hydrogenases, we used a molecular approach. Filamentous strains from a broad range of sources were screened for the presence of hup (uptake hydrogenase), xisC (rearrangement within hupL), and hox (bidirectional hydrogenase) genes. As expected, an uptake hydrogenase seems to be present in all N2-fixing cyanobacteria. On the other hand, no evidence was found for the presence of a conventional bidirectional enzyme in several strains. Similarly, the presence of xisC is not a characteristic shared by all the heterocyst-forming cyanobacteria. Although tempting, it is not possible to establish a correlation between the presence/absence of the bidirectional hydrogenase and the occurrence of xisC. The natural molecular variation of hydrogenases in cyanobacteria is certainly a field to explore, both to understand the physiological functions of the respective enzymes and to identify a genetic background to be used when constructing a strain for photobiological H2 production in a bioreactor. Springer-Verlag New York Inc., 2000 Tamagnini Paula Department of Physiological Botany, EBC, Uppsala University, Villavägen 6, S-752 36 Uppsala, Sweden, SE aut Costa José-Luís Department of Botany and Institute for Molecular and Cell Biology, University of Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal, PT aut Almeida Lígia Department of Botany and Institute for Molecular and Cell Biology, University of Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal, PT aut Oliveira Maria-José Department of Botany and Institute for Molecular and Cell Biology, University of Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal, PT aut Salema Roberto Department of Botany and Institute for Molecular and Cell Biology, University of Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal, PT aut Lindblad Peter Department of Physiological Botany, EBC, Uppsala University, Villavägen 6, S-752 36 Uppsala, Sweden, SE aut https://doi.org/10.1007/s002840010070 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010070 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tamagnini Paula Department of Physiological Botany, EBC, Uppsala University, Villavägen 6, S-752 36 Uppsala, Sweden, SE aut NATIONALLICENCE 700 1- Costa José-Luís Department of Botany and Institute for Molecular and Cell Biology, University of Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal, PT aut NATIONALLICENCE 700 1- Almeida Lígia Department of Botany and Institute for Molecular and Cell Biology, University of Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal, PT aut NATIONALLICENCE 700 1- Oliveira Maria-José Department of Botany and Institute for Molecular and Cell Biology, University of Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal, PT aut NATIONALLICENCE 700 1- Salema Roberto Department of Botany and Institute for Molecular and Cell Biology, University of Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal, PT aut NATIONALLICENCE 700 1- Lindblad Peter Department of Physiological Botany, EBC, Uppsala University, Villavägen 6, S-752 36 Uppsala, Sweden, SE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer