caa a22 4500 475745752 CHVBK 20180406123512.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s002849910040 doi (NATIONALLICENCE)springer-10.1007/s002849910040 The Crystal Proteins from Bacillus thuringiensis subsp. thompsoni Display a Synergistic Activity Against the Codling Moth, Cydia pomonella [Elektronische Daten] [Cécile Rang, Lawrence A. Lacey, Roger Frutos] Abstract.: Crystal proteins from Bacillus thuringiensis subsp. thompsoni strain HnC are active against the codling moth, Cydia pomonella, a major pest of orchards. Inclusion bodies purified from strain HnC displayed an LC50 of 3.34 × 10−3μg/μl. HnC-purified crystals were tenfold more active than Cry2Aa and Cry1Aa toxins, and 100-fold more toxic than Cry1Ab. The 34-kDa and 40-kDa proteins contained in HnC inclusion bodies were shown to act synergistically. The toxicity of crystal proteins produced by the recombinant B. thuringiensis strain BT-OP expressing the full-length native operon was about tenfold higher than that of the 34-kDa protein. When the gene encoding the non-insecticidal 40-kDa protein, which is not active, was introduced into the recombinant strain producing only the 34-kDa protein, the toxicity was raised tenfold and was similar to that of the strain BT-OP. Springer-Verlag New York Inc., 2000 Rang Cécile CIRAD, Avenue Agropolis, B.P. 5035, 34032 Montpellier Cedex 1, France, FR aut Lacey Lawrence A. USDA-ARS, Yakima Agricultural Research Laboratory, 5230 Konnowac Pass Road, Wapato, WA 98951, USA, US aut Frutos Roger CIRAD, Avenue Agropolis, B.P. 5035, 34032 Montpellier Cedex 1, France, FR aut https://doi.org/10.1007/s002849910040 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910040 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rang Cécile CIRAD, Avenue Agropolis, B.P. 5035, 34032 Montpellier Cedex 1, France, FR aut NATIONALLICENCE 700 1- Lacey Lawrence A. USDA-ARS, Yakima Agricultural Research Laboratory, 5230 Konnowac Pass Road, Wapato, WA 98951, USA, US aut NATIONALLICENCE 700 1- Frutos Roger CIRAD, Avenue Agropolis, B.P. 5035, 34032 Montpellier Cedex 1, France, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer