caa a22 4500 475745760 CHVBK 20180406123512.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s002849910031 doi (NATIONALLICENCE)springer-10.1007/s002849910031 Streptococcal Opacity Factor: A Family of Bifunctional Proteins with Lipoproteinase and Fibronectin-Binding Activities [Elektronische Daten] [Viacheslav Katerov, Per-Erik Lindgren, Artem A. Totolian, Claes Schalén] Abstract.: The serum opacity factor (SOF) of Streptococcus pyogenes is a type-specific lipoproteinase of unknown biological significance. We have sequenced the sof gene and characterized the corresponding SOF protein from a strain of type M63. It was found that sof63 is related to sof22 and that, similar to SOF22 [25], SOF63 binds fibronectin. Moreover, we demonstrate opacity factor activity in a Streptococcus dysgalactiae fibronectin-binding protein FnBA that is structurally related to the SOF proteins of S. pyogenes. Sequence analysis of these three SOF proteins showed a unique periodical pattern of conserved and variable regions. The enzymatically active part of SOF63 was localized to the fragment corresponding to the entire set of conserved and variable sequences, while for fibronectin-binding a single repeat in the C terminal part of the protein was sufficient. The results show that streptococcal SOF proteins form a novel family of bifunctional proteins with lipoproteinase and fibronectin-binding activities. Springer-Verlag New York Inc., 2000 Katerov Viacheslav Department of Infectious Diseases and Medical Microbiology, Lund University, Sölvegatan 23, S-22362 Lund, Sweden, SE aut Lindgren Per-Erik Department of Medical Microbiology, Linköping University, Linköping, Sweden, SE aut Totolian Artem A. Institute of Experimental Medicine, Academy of the Medical Sciences, St. Petersburg, Russia, RU aut Schalén Claes Department of Infectious Diseases and Medical Microbiology, Lund University, Sölvegatan 23, S-22362 Lund, Sweden, SE aut https://doi.org/10.1007/s002849910031 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910031 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Katerov Viacheslav Department of Infectious Diseases and Medical Microbiology, Lund University, Sölvegatan 23, S-22362 Lund, Sweden, SE aut NATIONALLICENCE 700 1- Lindgren Per-Erik Department of Medical Microbiology, Linköping University, Linköping, Sweden, SE aut NATIONALLICENCE 700 1- Totolian Artem A. Institute of Experimental Medicine, Academy of the Medical Sciences, St. Petersburg, Russia, RU aut NATIONALLICENCE 700 1- Schalén Claes Department of Infectious Diseases and Medical Microbiology, Lund University, Sölvegatan 23, S-22362 Lund, Sweden, SE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer