caa a22 4500 475745787 CHVBK 20180406123512.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s002849910030 doi (NATIONALLICENCE)springer-10.1007/s002849910030 Inhibition of Germ Tube Formation by Candida albicans by Local Anesthetics: An Effect Related to Ionic Channel Blockade [Elektronische Daten] [Acácio A.G. Rodrigues, Cidália Pina-Vaz, Per-Anders Mårdh, José Martinez-de-Oliveira, António Freitas-da-Fonseca] Abstract.: Formation of germ tubes by Candida albicans has been assumed as a putative virulence factor. Local anesthetics (LAs), e.g., lidocaine and bupivacaine, are known to inhibit germ tube formation. The study confirmed this observation for the novel drug ropivacaine, although it was less potent than the former two drugs. Hypothesizing that the effect is due to blockading ionic channels, we exposed Candida albicans to selective calcium blockers, i.e., nifedipine and verapamil, and to a general blocker of ionic channels, i.e., lanthanum. All blockers inhibited germ tube formation. The effect was dose-dependent and pH-independent. Addition of calcium reverted the effect of the blockers as well as the effect of lidocaine and ropivacaine. The study suggests that the inhibitory effect of LAs on germ tube formation by C. albicans is due to blockade of ionic channels, particularly calcium channels. Therefore, LAs can affect morphology and probably also the pathogenesis of C. albicans. Springer-Verlag New York Inc., 2000 Rodrigues Acácio A.G. Department of Microbiology, Alameda Prof. Hernani Monteiro, Porto School of Medicine, 4200 Porto, Portugal, PT aut Pina-Vaz Cidália Department of Microbiology, Alameda Prof. Hernani Monteiro, Porto School of Medicine, 4200 Porto, Portugal, PT aut Mårdh Per-Anders Department of Obstetrics and Gynecology, University Hospital, Lund, Sweden, SE aut Martinez-de-Oliveira José Department of Gynecology, Porto School of Medicine, University of Porto, Porto, Portugal, PT aut Freitas-da-Fonseca António Department of Microbiology, Alameda Prof. Hernani Monteiro, Porto School of Medicine, 4200 Porto, Portugal, PT aut https://doi.org/10.1007/s002849910030 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910030 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rodrigues Acácio A.G. Department of Microbiology, Alameda Prof. Hernani Monteiro, Porto School of Medicine, 4200 Porto, Portugal, PT aut NATIONALLICENCE 700 1- Pina-Vaz Cidália Department of Microbiology, Alameda Prof. Hernani Monteiro, Porto School of Medicine, 4200 Porto, Portugal, PT aut NATIONALLICENCE 700 1- Mårdh Per-Anders Department of Obstetrics and Gynecology, University Hospital, Lund, Sweden, SE aut NATIONALLICENCE 700 1- Martinez-de-Oliveira José Department of Gynecology, Porto School of Medicine, University of Porto, Porto, Portugal, PT aut NATIONALLICENCE 700 1- Freitas-da-Fonseca António Department of Microbiology, Alameda Prof. Hernani Monteiro, Porto School of Medicine, 4200 Porto, Portugal, PT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer