caa a22 4500 475745795 CHVBK 20180406123512.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s002849910042 doi (NATIONALLICENCE)springer-10.1007/s002849910042 Influence of Sulfate-Reducing Bacteria on Outdoor Hydrogen Production by Photosynthetic Bacterium with Seawater [Elektronische Daten] [Isamu Maeda, Tadashi Mizoguchi, Yoshiharu Miura, Kiyohito Yagi, Norio Shioji, Hitoshi Miyasaka] Abstract.: The application of seawater for bacterial fermentative production is a cost-effective technology. Hydrogen production by marine photosynthetic bacterium with seawater failed to continue after more than 10 days, and was accompanied by the formation of hydrogen sulfide and a change in culture color from red to black. However, substrate consumption in the blackish culture was comparable to that in a hydrogen-producing culture. A decrease in hydrogen production occurred upon the addition of sodium sulfide at concentrations of 1.5 mM or higher. PCR analysis targeted at the 16S rDNA sequence selective for sulfate-reducing bacteria revealed the existence of sulfate-reducing bacteria in inoculation cultures of the phototrophic bacterium and medium for hydrogen production. Hence, the high sulfate concentration of seawater, the low oxidation-reduction potential under hydrogen-producing conditions, and the presence of electron donors such as acetate might promote the metabolic activities of sulfate-reducing bacteria, resulting in the deterioration of hydrogen production with seawater. Springer-Verlag New York Inc., 2000 Maeda Isamu Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan, JP aut Mizoguchi Tadashi Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan, JP aut Miura Yoshiharu Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan, JP aut Yagi Kiyohito Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan, JP aut Shioji Norio Mitsubishi Heavy Industries Ltd., Takasago, Hyogo 676-0008, Japan, JP aut Miyasaka Hitoshi Kansai Electric Power Co. Amagasaki, Hyogo 661-0974, Japan, JP aut https://doi.org/10.1007/s002849910042 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910042 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Maeda Isamu Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan, JP aut NATIONALLICENCE 700 1- Mizoguchi Tadashi Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan, JP aut NATIONALLICENCE 700 1- Miura Yoshiharu Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan, JP aut NATIONALLICENCE 700 1- Yagi Kiyohito Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan, JP aut NATIONALLICENCE 700 1- Shioji Norio Mitsubishi Heavy Industries Ltd., Takasago, Hyogo 676-0008, Japan, JP aut NATIONALLICENCE 700 1- Miyasaka Hitoshi Kansai Electric Power Co. Amagasaki, Hyogo 661-0974, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer