caa a22 4500 475745817 CHVBK 20180406123513.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s002849910041 doi (NATIONALLICENCE)springer-10.1007/s002849910041 Occurrence of Bacillus thuringiensis on Cured Tobacco Leaves [Elektronische Daten] [Pascale Kaelin,, Ferruccio Gadani] Abstract.: A worldwide survey was conducted to evaluate the frequency and distribution of Bacillus thuringiensis populations on cured tobacco leaves during post-harvest storage. In total, 133 tobacco samples of different types and origins were analyzed. Nine percent of the samples showed the presence of B. thuringiensis, and 24 B. thuringiensis strains were isolated and characterized. The majority of the isolates produced bipyramidal crystals, and three fourths of them showed a second type of crystal protein (cuboidal or heterogeneous crystals). Only three isolates showed the rhomboidal crystal morphology characteristic of the anti-coleopteran B. thuringiensis subsp. tenebrionis. PCR analysis with primers specific for cry1 and cry3 genes revealed eight distinct cry gene profiles. The results of this study indicate that B. thuringiensis is naturally present at low frequency on the phylloplane of cured tobacco leaves and that its distribution is worldwide. Springer-Verlag New York Inc., 2000 Kaelin, Pascale Research & Development Dept., Philip Morris Europe, CH-2003 Neuchâtel, Switzerland, CH aut Gadani Ferruccio Research & Development Dept., Philip Morris Europe, CH-2003 Neuchâtel, Switzerland, CH aut https://doi.org/10.1007/s002849910041 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910041 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kaelin Pascale Research & Development Dept., Philip Morris Europe, CH-2003 Neuchâtel, Switzerland, CH aut NATIONALLICENCE 700 1- Gadani Ferruccio Research & Development Dept., Philip Morris Europe, CH-2003 Neuchâtel, Switzerland, CH aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer