caa a22 4500 475745833 CHVBK 20180406123513.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s002849910036 doi (NATIONALLICENCE)springer-10.1007/s002849910036 Purification and Characterization of Invertase from Lactobacillus reuteri CRL 1100 [Elektronische Daten] [Silvia Cuezzo de Ginés, María C. Maldonado, Graciela Font de Valdez] Abstract.: The invertase of Lactobacillus reuteri CRL 1100 is a glycoprotein composed by a single subunit with a molecular weight of 58 kDa. The enzyme was stable below 45°C over a wide pH range (4.5-7.0) with maximum activity at pH 6.0 and 37°C. The invertase activity was significantly inhibited by bivalent metal ions (Ca++, Cu++, Cd++, and Hg++), β-mercaptoethanol, and dithiothreitol and partially improved by ethylenediaminetetraacetic acid. The enzyme was purified 32 times over the crude extract by gel filtration and ion-exchange chromatography with a recovery of 17%. The K m and Vmax values for sucrose were 6.66 mM and 0.028 μmol/min, respectively. An invertase is purified and characterized for the first time in Lactobacillus, and it proved to be a β-fructofuranosidase. Springer-Verlag New York Inc., 2000 Cuezzo de Ginés Silvia Cátedra de Bioquímica Clínica III, Universidad Nacional de Tucumán, Argentina, AR aut Maldonado María C. Cátedra de Microbiología Industrial, Universidad Nacional de Tucumán, Argentina, AR aut Font de Valdez Graciela Cátedra de Microbiología Superior, Universidad Nacional de Tucumán, Argentina, AR aut https://doi.org/10.1007/s002849910036 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910036 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cuezzo de Ginés Silvia Cátedra de Bioquímica Clínica III, Universidad Nacional de Tucumán, Argentina, AR aut NATIONALLICENCE 700 1- Maldonado María C. Cátedra de Microbiología Industrial, Universidad Nacional de Tucumán, Argentina, AR aut NATIONALLICENCE 700 1- Font de Valdez Graciela Cátedra de Microbiología Superior, Universidad Nacional de Tucumán, Argentina, AR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer