caa a22 4500 475745876 CHVBK 20180406123513.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s002849910034 doi (NATIONALLICENCE)springer-10.1007/s002849910034 Mcchs1 , a Member of a Chitin Synthase Gene Family in Mucor circinelloides , Is Differentially Expressed During Dimorphism [Elektronische Daten] [M. Angeles López-Matas,, Arturo P. Eslava, José María Díaz-Mínguez] Abstract.: A complete chitin synthase gene and one chitin synthase gene fragment of the zygomycete Mucor circinelloides have been cloned and analyzed. Both genes encode zymogenic Class II chitin synthases. Hybridization analysis showed that there must exist at least another Class II chitin synthase gene in M. circinelloides highly homologous to the cloned Mcchs1 and Mcchs2. The expression of these genes during the dimorphic growing stages was analyzed. Northern hybridizations showed that Mcchs1 transcript accumulates only during the exponentially growing hyphal stage, while no expression could be detected in the yeast form. Expression of Mcchs2 could not be detected at any stage. Accumulation of Mcchs1 transcript was not influenced by visible light. The existence of a multigene chitin synthase family and the observation that Mcchs1 transcription depends upon the dimorphic stage indicate that various chitin synthase activities may have different roles in the dimorphic growth of M. circinelloides. Springer-Verlag New York Inc., 2000 López-Matas, M. Angeles Area de Genética, Departamento de Microbiología y Genética, Universidad de Salamanca, Plaza de los Doctores de la Reina s/n, 37007 Salamanca, Spain, ES aut Eslava Arturo P. Area de Genética, Departamento de Microbiología y Genética, Universidad de Salamanca, Plaza de los Doctores de la Reina s/n, 37007 Salamanca, Spain, ES aut Díaz-Mínguez José María Area de Genética, Departamento de Microbiología y Genética, Universidad de Salamanca, Plaza de los Doctores de la Reina s/n, 37007 Salamanca, Spain, ES aut https://doi.org/10.1007/s002849910034 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910034 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- López-Matas M. Angeles Area de Genética, Departamento de Microbiología y Genética, Universidad de Salamanca, Plaza de los Doctores de la Reina s/n, 37007 Salamanca, Spain, ES aut NATIONALLICENCE 700 1- Eslava Arturo P. Area de Genética, Departamento de Microbiología y Genética, Universidad de Salamanca, Plaza de los Doctores de la Reina s/n, 37007 Salamanca, Spain, ES aut NATIONALLICENCE 700 1- Díaz-Mínguez José María Area de Genética, Departamento de Microbiología y Genética, Universidad de Salamanca, Plaza de los Doctores de la Reina s/n, 37007 Salamanca, Spain, ES aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer