caa a22 4500 475745906 CHVBK 20180406123513.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s002849910044 doi (NATIONALLICENCE)springer-10.1007/s002849910044 Occurrence of Bacillus thuringiensis in Fresh Waters of Japan [Elektronische Daten] [Tokio Ichimatsu, Eiichi Mizuki, Katsumi Nishimura, Tetsuyuki Akao, Hiroyuki Saitoh, Kazuhiko Higuchi, Michio Ohba] Abstract.: Bacillus thuringiensis was recovered at a relatively high frequency from both running and still fresh waters in natural environments of Kyushu, Japan. Of 107 water samples examined, 53 (49.5%) contained this organism. The frequency of B. thuringiensis colonies was 4.4% among 4414 colonies of the Bacillus cereus/B. thuringiensis group. The density of this bacterium in fresh waters averaged 0.45 cfu/ml. Serologically, B. thuringiensis isolates were assigned to 26 H serotypes. Of these, H14/36 (H serovar israelensis/malaysiensis) was the predominant, followed by the serotypes H3abc (kurstaki), H27 (mexicanensis), H3ad (sumiyoshiensis), and H35 (seoulensis). Of 195 isolates, 52 (26.7%) exhibited larvicidal activity against aquatic Diptera; 21 killed Culex pipiens molestus (Culicidae) only, and 31 were active on both the culicine mosquito and the moth-fly, Clogmia albipunctata (Psychodidae). The Diptera-toxic isolates produced spherical or irregularly pointed parasporal inclusions. Springer-Verlag New York Inc., 2000 Ichimatsu Tokio Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa, Kurume, Fukuoka 839-0861, Japan, JP aut Mizuki Eiichi Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa, Kurume, Fukuoka 839-0861, Japan, JP aut Nishimura Katsumi Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa, Kurume, Fukuoka 839-0861, Japan, JP aut Akao Tetsuyuki Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa, Kurume, Fukuoka 839-0861, Japan, JP aut Saitoh Hiroyuki Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa, Kurume, Fukuoka 839-0861, Japan, JP aut Higuchi Kazuhiko Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa, Kurume, Fukuoka 839-0861, Japan, JP aut Ohba Michio Institute of Biological Control, Kyushu University, Fukuoka 812-8581, Japan, JP aut https://doi.org/10.1007/s002849910044 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910044 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ichimatsu Tokio Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa, Kurume, Fukuoka 839-0861, Japan, JP aut NATIONALLICENCE 700 1- Mizuki Eiichi Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa, Kurume, Fukuoka 839-0861, Japan, JP aut NATIONALLICENCE 700 1- Nishimura Katsumi Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa, Kurume, Fukuoka 839-0861, Japan, JP aut NATIONALLICENCE 700 1- Akao Tetsuyuki Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa, Kurume, Fukuoka 839-0861, Japan, JP aut NATIONALLICENCE 700 1- Saitoh Hiroyuki Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa, Kurume, Fukuoka 839-0861, Japan, JP aut NATIONALLICENCE 700 1- Higuchi Kazuhiko Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa, Kurume, Fukuoka 839-0861, Japan, JP aut NATIONALLICENCE 700 1- Ohba Michio Institute of Biological Control, Kyushu University, Fukuoka 812-8581, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer