caa a22 4500 475745973 CHVBK 20180406123513.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s002849910053 doi (NATIONALLICENCE)springer-10.1007/s002849910053 Uniparental Mitochondrial Transmission in Sexual Crosses in Cryptococcus neoformans [Elektronische Daten] [Jianping Xu, Rabia Y. Ali, Devon A. Gregory, Denise Amick, Sarah E. Lambert, Heather J. Yoell, Rytas J. Vilgalys, Thomas G. Mitchell] Abstract.: Restriction fragment length polymorphism (RFLP) in the large ribosomal RNA region of the mitochondrial DNA (mtDNA) was developed as a genetic marker for investigating mitochondrial transmission in sexual crosses of the human pathogenic basidiomycetous yeast Cryptococcus neoformans. Strain JEC20 of C. neoformans var. neoformans (mat a) was mated with six strains of C. neoformans var. grubii (mat alpha). Successful mating was indicated by the formation of hyphae and basidiospores. These basidiospores were examined for mtDNA RFLP genotypes. All 570 basidiospores examined from the six crosses showed the mtDNA genotype of strain JEC20. The failure to recover the C. neoformans var. grubii mtDNA in any cross indicates that the C. neoformans var. grubii mtDNA is either selectively eliminated in the newly formed dikaryon or selectively excluded in the immediate dikaryotic hyphae of the newly formed dikaryon. Springer-Verlag New York Inc., 2000 Xu Jianping Department of Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710, USA, US aut Ali Rabia Y. Department of Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710, USA, US aut Gregory Devon A. Department of Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710, USA, US aut Amick Denise Department of Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710, USA, US aut Lambert Sarah E. Department of Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710, USA, US aut Yoell Heather J. Department of Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710, USA, US aut Vilgalys Rytas J. Department of Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710, USA, US aut Mitchell Thomas G. Department of Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710, USA, US aut https://doi.org/10.1007/s002849910053 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910053 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Jianping Department of Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710, USA, US aut NATIONALLICENCE 700 1- Ali Rabia Y. Department of Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710, USA, US aut NATIONALLICENCE 700 1- Gregory Devon A. Department of Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710, USA, US aut NATIONALLICENCE 700 1- Amick Denise Department of Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710, USA, US aut NATIONALLICENCE 700 1- Lambert Sarah E. Department of Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710, USA, US aut NATIONALLICENCE 700 1- Yoell Heather J. Department of Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710, USA, US aut NATIONALLICENCE 700 1- Vilgalys Rytas J. Department of Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710, USA, US aut NATIONALLICENCE 700 1- Mitchell Thomas G. Department of Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer