caa a22 4500 475746007 CHVBK 20180406123513.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s002849910056 doi (NATIONALLICENCE)springer-10.1007/s002849910056 HSP16.6 Is Involved in the Development of Thermotolerance and Thylakoid Stability in the Unicellular Cyanobacterium, Synechocystis sp. PCC 6803 [Elektronische Daten] [Sengyong Lee, Heather A. Owen, Daniel J. Prochaska, Susan R. Barnum] Abstract.: The low molecular weight (LMW) heat shock protein (HSP), HSP16.6, in the unicellular cyanobacterium, Synechocystis sp. PCC 6803, protects cells from elevated temperatures. A 95% reduction in the survival of mutant cells with an inactivated hsp16.6 was observed after exposure for 1 h at 47°C. Wild-type cell survival was reduced to only 41%. HSP16.6 is also involved in the development of thermotolerance. After a sublethal heat shock at 43°C for 1 h and subsequent challenge exposure at 49°C for 40 min, mutant cells did not survive, while 64% of wild-type cells survived. Ultrastructural changes in the integrity of thylakoid membranes of heat-shocked mutant cells also are discussed. These results demonstrate an important protective role for HSP16.6 in the protection of cells and, in particular, thylakoid membrane against thermal stress. Springer-Verlag New York Inc., 2000 Lee Sengyong Department of Botany, Miami University, Oxford, OH 45056, USA, US aut Owen Heather A. Electron Microscopy Laboratory, Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 53201, USA, US aut Prochaska Daniel J. Department of Botany, Miami University, Oxford, OH 45056, USA, US aut Barnum Susan R. Department of Botany, Miami University, Oxford, OH 45056, USA, US aut https://doi.org/10.1007/s002849910056 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910056 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Sengyong Department of Botany, Miami University, Oxford, OH 45056, USA, US aut NATIONALLICENCE 700 1- Owen Heather A. Electron Microscopy Laboratory, Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 53201, USA, US aut NATIONALLICENCE 700 1- Prochaska Daniel J. Department of Botany, Miami University, Oxford, OH 45056, USA, US aut NATIONALLICENCE 700 1- Barnum Susan R. Department of Botany, Miami University, Oxford, OH 45056, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer