caa a22 4500 475746015 CHVBK 20180406123513.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s002849910052 doi (NATIONALLICENCE)springer-10.1007/s002849910052 Molecular Characterization of the Leucine Plasmid from Buchnera aphidicola , Primary Endosymbiont of the Aphid Acyrthosiphon pisum [Elektronische Daten] [Teresa Soler, Amparo Latorre, Beatriz Sabater, Francisco J. Silva] Abstract.: The complete sequence of the leucine plasmid of Buchnera aphidicola from the aphid Acyrthosiphon pisum (pLeu-BAp) is reported. Its gene organization was concordant with those of other leucine plasmids of Buchnera from aphids of the Aphidini and Macrosiphini tribes. Three inverted repeats are present in pLeu-BAp. Two of them are also present in pLeu from the family Aphididae: (i) SIR1, located downstream the leucine operon, resembles a rho-independent terminator of transcription, and (ii) LIR1, located upstream of the leucine operon, is suggested to be involved in transcription termination or messenger stability. The third, located near the putative ATGC repeats involved in the origin of replication, is specific in aphids of the Macrosiphini tribe. Phylogenetic analyses based on sequences of leuA, leuB, leuC, leuD, repA1 and ORF1 showed a closer relationship between Buchnera (A. pisum) and Buchnera (Diuraphis noxia). However, tree topologies indicate that the split between both aphid species took place soon after the formation of the Macrosiphini lineage. Springer-Verlag New York Inc., 2000 Soler Teresa Institut Cavanilles de Biodiversitat i Biologia Evolutiva and Departament de Genètica, Universitat de València, Apartat 22085, 46071 València, Spain, ES aut Latorre Amparo Institut Cavanilles de Biodiversitat i Biologia Evolutiva and Departament de Genètica, Universitat de València, Apartat 22085, 46071 València, Spain, ES aut Sabater Beatriz Institut Cavanilles de Biodiversitat i Biologia Evolutiva and Departament de Genètica, Universitat de València, Apartat 22085, 46071 València, Spain, ES aut Silva Francisco J. Institut Cavanilles de Biodiversitat i Biologia Evolutiva and Departament de Genètica, Universitat de València, Apartat 22085, 46071 València, Spain, ES aut https://doi.org/10.1007/s002849910052 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910052 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Soler Teresa Institut Cavanilles de Biodiversitat i Biologia Evolutiva and Departament de Genètica, Universitat de València, Apartat 22085, 46071 València, Spain, ES aut NATIONALLICENCE 700 1- Latorre Amparo Institut Cavanilles de Biodiversitat i Biologia Evolutiva and Departament de Genètica, Universitat de València, Apartat 22085, 46071 València, Spain, ES aut NATIONALLICENCE 700 1- Sabater Beatriz Institut Cavanilles de Biodiversitat i Biologia Evolutiva and Departament de Genètica, Universitat de València, Apartat 22085, 46071 València, Spain, ES aut NATIONALLICENCE 700 1- Silva Francisco J. Institut Cavanilles de Biodiversitat i Biologia Evolutiva and Departament de Genètica, Universitat de València, Apartat 22085, 46071 València, Spain, ES aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer