caa a22 4500 47574604X CHVBK 20180406123513.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s002849910064 doi (NATIONALLICENCE)springer-10.1007/s002849910064 Coexpression of cyt1Aa of Bacillus thuringiensis subsp. israelensis with Bacillus sphaericus Binary Toxin Gene in Acrystalliferous Strain of B. thuringiensis [Elektronische Daten] [Tianyong Li, Fan Sun, Zhiming Yuan, Yongmei Zhang, Jianxiu Yu, Yi Pang] Abstract.: The cyt1Aa gene of Bacillus thuringiensis subsp. israelensis and binary toxin gene of Bacillus sphaericus C3-41 were introduced into an acrystalliferous strain of B. thuringiensis independently and in combination by using shuttle vector pBU4. SDS-PAGE and Western blot analysis proved that cyt1Aa and binary toxin genes coexpressed during the sporulation of the recombinant. Transformant strain expressing the Cyt1Aa and binary toxin proteins in combination was more toxic to susceptible and resistant Culex pipiens quinquefasciatus than the transformants expressing Cyt1Aa protein or binary toxin proteins independently. It was suggested that large amount of production of Cyt1Aa protein and binary toxin proteins possibly interacted synergistically, thereby increasing its mosquitocidal toxicity significantly. Springer-Verlag New York Inc., 2000 Li Tianyong Wuhan Institute of Virology, Academia Sinica, Wuhan 430071, China, CN aut Sun Fan Wuhan Institute of Virology, Academia Sinica, Wuhan 430071, China, CN aut Yuan Zhiming Wuhan Institute of Virology, Academia Sinica, Wuhan 430071, China, CN aut Zhang Yongmei Wuhan Institute of Virology, Academia Sinica, Wuhan 430071, China, CN aut Yu Jianxiu The State Key Laboratory of Biocontrol, Zhongshan University, Guangzhou 510275, China, CN aut Pang Yi The State Key Laboratory of Biocontrol, Zhongshan University, Guangzhou 510275, China, CN aut https://doi.org/10.1007/s002849910064 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910064 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Tianyong Wuhan Institute of Virology, Academia Sinica, Wuhan 430071, China, CN aut NATIONALLICENCE 700 1- Sun Fan Wuhan Institute of Virology, Academia Sinica, Wuhan 430071, China, CN aut NATIONALLICENCE 700 1- Yuan Zhiming Wuhan Institute of Virology, Academia Sinica, Wuhan 430071, China, CN aut NATIONALLICENCE 700 1- Zhang Yongmei Wuhan Institute of Virology, Academia Sinica, Wuhan 430071, China, CN aut NATIONALLICENCE 700 1- Yu Jianxiu The State Key Laboratory of Biocontrol, Zhongshan University, Guangzhou 510275, China, CN aut NATIONALLICENCE 700 1- Pang Yi The State Key Laboratory of Biocontrol, Zhongshan University, Guangzhou 510275, China, CN aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer