caa a22 4500 475746074 CHVBK 20180406123513.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s002849910063 doi (NATIONALLICENCE)springer-10.1007/s002849910063 Transcriptional Analysis of Hydrogenase Genes in the Cyanobacteria Anacystis nidulans and Anabaena variabilis Monitored by RT-PCR [Elektronische Daten] [Gudrun Boison, Hermann Bothe, Oliver Schmitz] Abstract.: Diverse cyanobacteria express an uptake hydrogenase, encoded by the genes hupSL, and a bidirectional, NAD(P)+-reducing hydrogenase with the genes hox(E)FUYH. In the unicellular Anacystis nidulans, the hox genes are organized on two separate loci, whereas they are contiguous in one cluster, though interspersed with two unidentified reading frames, ORF 3 and 8, in the heterocystous Anabaena variabilis. The hox gene clusters of these two cyanobacteria have now been transcriptionally analyzed by RT-PCR. A polycistronic transcript was identified in both cyanobacteria. In A. nidulans, one message for each locus has been detected, the dicistronic hoxEF unit, and the polycistronic hoxUYHWhypAB one. In A. variabilis, the transcript consists of the hox genes hoxFUYH as well as the unidentified ORFs. Previous enzyme determinations on the occurrence of the uptake hydrogenase in vegetative cells and thus outside of heterocysts gave ambiguous results. Therefore, transcription of both hup and hox genes has been analyzed in both heterocysts and vegetative cells of A. variabilis. A hupL transcript is detectable in heterocysts and also, though less extensive but clearly discernible, in vegetative cells of NH4 +-grown A. variabilis. Springer-Verlag New York Inc., 2000 Boison Gudrun Botanisches Institut, Universität zu Köln, Gyrhofstr. 15, D-50923 Köln, Germany, DE aut Bothe Hermann Botanisches Institut, Universität zu Köln, Gyrhofstr. 15, D-50923 Köln, Germany, DE aut Schmitz Oliver Botanisches Institut, Universität zu Köln, Gyrhofstr. 15, D-50923 Köln, Germany, DE aut https://doi.org/10.1007/s002849910063 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910063 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Boison Gudrun Botanisches Institut, Universität zu Köln, Gyrhofstr. 15, D-50923 Köln, Germany, DE aut NATIONALLICENCE 700 1- Bothe Hermann Botanisches Institut, Universität zu Köln, Gyrhofstr. 15, D-50923 Köln, Germany, DE aut NATIONALLICENCE 700 1- Schmitz Oliver Botanisches Institut, Universität zu Köln, Gyrhofstr. 15, D-50923 Köln, Germany, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer