caa a22 4500 475746082 CHVBK 20180406123513.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s002849910065 doi (NATIONALLICENCE)springer-10.1007/s002849910065 Isolation and Identification of Ruminal Methanogens from Grazing Cattle [Elektronische Daten] [Graeme N. Jarvis, Carsten Strömpl, Diana M. Burgess, Lucy C. Skillman, Edward R. B. Moore, Keith N. Joblin] Abstract.: To obtain information on the diversity of ruminal methanogens in grazing animals, three ruminal methanogens from grazing cattle were characterized and identified. Two of the isolates were rod-shaped, with one staining Gram-positive and being non-motile (BRM9), and the other (BRM16) staining Gram-negative and being motile. These isolates grew only on H2/CO2 and formate, and optimally at 38°C and pH 6.5-7.0. The third isolate (CM1) was non-motile, pseudosarcina-shaped, and grew on H2/CO2, acetate, and methyl-containing compounds, with optimal growth at 40°C and pH 6.5. DNA was prepared from the three isolates, and their 16S rRNA genes were sequenced. Phenotypic data and comparisons of nearly complete 16S rDNA sequences showed that BRM9, BRM16, and CM1 are strains of Methanobacterium formicicum, Methanomicrobium mobile, and Methanosarcina barkeri respectively. To the best of our knowledge, this is the first information on ruminal methanogens in cattle maintained under grazing management. Springer-Verlag New York Inc., 2000 Jarvis Graeme N. Rumen Microbiology Unit, AgResearch Grasslands Research Centre, Private Bag 11008, Palmerston North, New Zealand, NZ aut Strömpl Carsten Division of Microbiology, GBFmbH-National Research Center for Biotechnology, Mascheroder Weg 1, Braunschweig 38124, Germany, DE aut Burgess Diana M. Rumen Microbiology Unit, AgResearch Grasslands Research Centre, Private Bag 11008, Palmerston North, New Zealand, NZ aut Skillman Lucy C. Rumen Microbiology Unit, AgResearch Grasslands Research Centre, Private Bag 11008, Palmerston North, New Zealand, NZ aut Moore Edward R. B. Division of Microbiology, GBFmbH-National Research Center for Biotechnology, Mascheroder Weg 1, Braunschweig 38124, Germany, DE aut Joblin Keith N. Rumen Microbiology Unit, AgResearch Grasslands Research Centre, Private Bag 11008, Palmerston North, New Zealand, NZ aut https://doi.org/10.1007/s002849910065 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910065 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jarvis Graeme N. Rumen Microbiology Unit, AgResearch Grasslands Research Centre, Private Bag 11008, Palmerston North, New Zealand, NZ aut NATIONALLICENCE 700 1- Strömpl Carsten Division of Microbiology, GBFmbH-National Research Center for Biotechnology, Mascheroder Weg 1, Braunschweig 38124, Germany, DE aut NATIONALLICENCE 700 1- Burgess Diana M. Rumen Microbiology Unit, AgResearch Grasslands Research Centre, Private Bag 11008, Palmerston North, New Zealand, NZ aut NATIONALLICENCE 700 1- Skillman Lucy C. Rumen Microbiology Unit, AgResearch Grasslands Research Centre, Private Bag 11008, Palmerston North, New Zealand, NZ aut NATIONALLICENCE 700 1- Moore Edward R. B. Division of Microbiology, GBFmbH-National Research Center for Biotechnology, Mascheroder Weg 1, Braunschweig 38124, Germany, DE aut NATIONALLICENCE 700 1- Joblin Keith N. Rumen Microbiology Unit, AgResearch Grasslands Research Centre, Private Bag 11008, Palmerston North, New Zealand, NZ aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer