caa a22 4500 475746139 CHVBK 20180406123514.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s002849910020 doi (NATIONALLICENCE)springer-10.1007/s002849910020 Production of 10-Ketostearic Acid and 10-Hydroxystearic Acid by Strains of Sphingobacterium thalpophilum Isolated from Composted Manure [Elektronische Daten] [Tsung Min Kuo, Alan C. Lanser, Larry K. Nakamura, Ching T. Hou] Abstract.: Six strains of Sphingobacterium thalpophilum were isolated from a compost mixture enriched with oleic acid. These strains converted oleic acid to 10-ketostearic acid (10-KSA; 87-94% of the total conversion product) and to 10-hydroxystearic acid (10-HSA; 6-13%) exhibiting three levels of total product yields. The predominant production of 10-KSA by these new S. thalpophilum isolates is in contrast to strain 142b (NRRL B-14797) previously isolated from a commercial compost, which produces exclusively 10-HSA. The production yield of greater than 75% 10-KSA was achieved in 36 h, acting on 0.26 g of oleic acid in 30-ml fermentation broth incubated with agitation at 28°C. For easy maintenance, fast-growth, and high bioreactivity, these S. thalpophilum strains are suited for developing a large-scale production of 10-KSA and 10-HSA. Springer-Verlag New York Inc., 2000 Kuo Tsung Min Oil Chemical Research, National Center for Agricultural Utilization Research, USDA-ARS, 1815 North University Street, Peoria, IL 61604, USA, US aut Lanser Alan C. Oil Chemical Research, National Center for Agricultural Utilization Research, USDA-ARS, 1815 North University Street, Peoria, IL 61604, USA, US aut Nakamura Larry K. Microbial Properties Research, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL 61604, USA, US aut Hou Ching T. Oil Chemical Research, National Center for Agricultural Utilization Research, USDA-ARS, 1815 North University Street, Peoria, IL 61604, USA, US aut https://doi.org/10.1007/s002849910020 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910020 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kuo Tsung Min Oil Chemical Research, National Center for Agricultural Utilization Research, USDA-ARS, 1815 North University Street, Peoria, IL 61604, USA, US aut NATIONALLICENCE 700 1- Lanser Alan C. Oil Chemical Research, National Center for Agricultural Utilization Research, USDA-ARS, 1815 North University Street, Peoria, IL 61604, USA, US aut NATIONALLICENCE 700 1- Nakamura Larry K. Microbial Properties Research, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL 61604, USA, US aut NATIONALLICENCE 700 1- Hou Ching T. Oil Chemical Research, National Center for Agricultural Utilization Research, USDA-ARS, 1815 North University Street, Peoria, IL 61604, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer