caa a22 4500 47574618X CHVBK 20180406123514.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s002849910025 doi (NATIONALLICENCE)springer-10.1007/s002849910025 Spherical Parasporal Inclusions of the Lepidoptera-Specific and Coleoptera-Specific Bacillus thuringiensis Strains: A Comparative Electron Microscopic Study [Elektronische Daten] [Naoya Wasano, Chisa Yasunaga-Aoki, Ryoichi Sato, Michio Ohba, Takeshi Kawarabata, Hidenori Iwahana] Abstract.: Four Lepidoptera-specific Bacillus thuringiensis strains that belong to the four H serogroups (serovars sumiyoshiensis, fukuokaensis, darmstadiensis, and japonensis) and a Coleoptera (Scarabaeidae)-specific strain belonging to serovar japonensis were examined for comparative ultrastructure of spherical parasporal inclusions. The prominent feature of the inclusions of the Lepidoptera-specific strains was the existence of thick, highly electron-dense envelopes surrounding a homogeneous protein matrix. The envelopes were 15.0-66.7 nm thick and consisted of 5-12 layers of membrane. This is also the case with inclusions of a Coleoptera-specific strain. The ultrastructure of inclusions from the five strains was in marked contrast to that of the bipyramidal parasporal inclusions produced by a Lepidoptera-specific serovar sotto strain. Springer-Verlag New York Inc., 2000 Wasano Naoya Institute of Biological Control, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan, JP aut Yasunaga-Aoki Chisa Institute of Biological Control, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan, JP aut Sato Ryoichi Graduate School of Bio-Applications and Systems Engineering, Koganei, Tokyo University of Agriculture and Technology, Tokyo 184-0012, Japan, JP aut Ohba Michio Institute of Biological Control, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan, JP aut Kawarabata Takeshi Institute of Biological Control, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan, JP aut Iwahana Hidenori Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-0054, Japan, JP aut https://doi.org/10.1007/s002849910025 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910025 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wasano Naoya Institute of Biological Control, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan, JP aut NATIONALLICENCE 700 1- Yasunaga-Aoki Chisa Institute of Biological Control, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan, JP aut NATIONALLICENCE 700 1- Sato Ryoichi Graduate School of Bio-Applications and Systems Engineering, Koganei, Tokyo University of Agriculture and Technology, Tokyo 184-0012, Japan, JP aut NATIONALLICENCE 700 1- Ohba Michio Institute of Biological Control, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan, JP aut NATIONALLICENCE 700 1- Kawarabata Takeshi Institute of Biological Control, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan, JP aut NATIONALLICENCE 700 1- Iwahana Hidenori Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-0054, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer