caa a22 4500 475746228 CHVBK 20180406123514.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s002849910024 doi (NATIONALLICENCE)springer-10.1007/s002849910024 A Replication Origin of Bacillus thuringiensis [Elektronische Daten] [Jianxiu Yu, Yingjie Zhang, Yi Pang, Minqing Xu] Abstract.: A replication origin of Bacillus thuringiensis (Bt) was found in a Bacillus thuringiensis-Escherichia coli shuttle vector of pHT3101. Deletion analysis showed that the replication origin was segregationally stable at suitable temperature for Bt growth. The fragment containing the replication origin was cloned in pUC18 and sequenced. It was 261 base pairs in length, located in the open reading frame 2 (ORF2) of BTSPB sequence. The 261-bp fragment was cloned in pBR322, creating an improved Bt-E. coli shuttle vector pBR261, which contained two resistance genes responsible for ampicillin and tetracycline. Our study showed that the replication origin structure could be recognized by replication protein of host cells. Springer-Verlag New York Inc., 2000 Yu Jianxiu State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, Guangzhou 510275, P.R. China, CN aut Zhang Yingjie State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, Guangzhou 510275, P.R. China, CN aut Pang Yi State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, Guangzhou 510275, P.R. China, CN aut Xu Minqing State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, Guangzhou 510275, P.R. China, CN aut https://doi.org/10.1007/s002849910024 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910024 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yu Jianxiu State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, Guangzhou 510275, P.R. China, CN aut NATIONALLICENCE 700 1- Zhang Yingjie State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, Guangzhou 510275, P.R. China, CN aut NATIONALLICENCE 700 1- Pang Yi State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, Guangzhou 510275, P.R. China, CN aut NATIONALLICENCE 700 1- Xu Minqing State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, Guangzhou 510275, P.R. China, CN aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer