caa a22 4500 475746236 CHVBK 20180406123514.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s002849910029 doi (NATIONALLICENCE)springer-10.1007/s002849910029 Organization and Sequence of Histidine Biosynthesis Genes hisH , -A , -F , and -IE in Thermoanaerobacter ethanolicus [Elektronische Daten] [Milutin Erbeznik, Herbert J. Strobel, Karl A. Dawson] Abstract.: Nucleotide sequence analysis of a 3.5-kb chromosomal fragment from the low G + C Gram-positive bacterium Thermoanaerobacter ethanolicus revealed a cluster of five contiguous open reading frames (ORFs) designated hisH, hisA, hisF, hisIE, and ORF5. The first four ORFs showed homology to genes of the histidine biosynthesis pathway, and ORF5 encoded a product with no significant similarities to polypeptides presently known. The hisH ORF was partial (truncated by cloning) and ORF5 was adjacent to xylF, which codes for a xylose-binding periplasmic protein. The five genes encoded putative proteins of >104, 237, 254, 216, and 169 amino acids, respectively. Amino acid sequence comparison of the four his gene products indicated closely related homologs in prokaryotes, varying from low G + C Gram-positive bacteria to archaea. This is the first report of his anabolic genes in a thermophilic anaerobic bacterium. Springer-Verlag New York Inc., 2000 Erbeznik Milutin Department of Animal Science, University of Kentucky, Lexington, KY 40546-0215, USA, US aut Strobel Herbert J. Department of Animal Science, University of Kentucky, Lexington, KY 40546-0215, USA, US aut Dawson Karl A. Department of Animal Science, University of Kentucky, Lexington, KY 40546-0215, USA, US aut https://doi.org/10.1007/s002849910029 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910029 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Erbeznik Milutin Department of Animal Science, University of Kentucky, Lexington, KY 40546-0215, USA, US aut NATIONALLICENCE 700 1- Strobel Herbert J. Department of Animal Science, University of Kentucky, Lexington, KY 40546-0215, USA, US aut NATIONALLICENCE 700 1- Dawson Karl A. Department of Animal Science, University of Kentucky, Lexington, KY 40546-0215, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer