caa a22 4500 475746244 CHVBK 20180406123514.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s002849910023 doi (NATIONALLICENCE)springer-10.1007/s002849910023 Overexpression of the cat-86 Gene Is Associated with Thermosensitivity in Bacillus subtilis [Elektronische Daten] [S. Marvin Friedman, Saikrishna Chatakondu, Diane Urbanski, Rui Lopes] Abstract.: Bacillus subtilis harboring the cat-86 constitutive plasmid pPL708C2 with an ochre mutation at the 9th codon (terc 9) was sensitive to chloramphenicol (Cms) and exhibited relative thermostability when heated at 47°C. Reversion to chloramphenicol resistance (Cmr) occurred at a frequency of 5.4 × 10−8. All of the plasmid Cmr revertants tested were thermosensitive. Similarly, wild-type pPL708C2 present in B. subtilis also rendered the bacterium thermosensitive. When a nonsense mutation is introduced at codon 141, however, this terc 141 variant of pPL708C2 failed to thermosensitize B. subtilis. Another variant of pPL708C2 that produces intact yet catalytically inactive CAT-86 has both His-16 and His-17 at the active site replaced by Pro. Nevertheless, cells of B. subtilis carrying this variant were thermosensitive. Plasmid-free and pPL708C2-bearing strains did not exhibit differences in major heat shock proteins. Electron micrographs revealed a threefold increase of inclusion bodies present in a strain harboring pPL708C2 when compared with those in an isogenic plasmid-free strain. Springer-Verlag New York Inc., 2000 Friedman S. Marvin Department of Biological Sciences, Hunter College of The City University of New York, 695 Park Avenue, New York, New York 10021, USA, US aut Chatakondu Saikrishna Department of Biological Sciences, Hunter College of The City University of New York, 695 Park Avenue, New York, New York 10021, USA, US aut Urbanski Diane Department of Biological Sciences, Hunter College of The City University of New York, 695 Park Avenue, New York, New York 10021, USA, US aut Lopes Rui Department of Biological Sciences, Hunter College of The City University of New York, 695 Park Avenue, New York, New York 10021, USA, US aut https://doi.org/10.1007/s002849910023 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910023 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Friedman S. Marvin Department of Biological Sciences, Hunter College of The City University of New York, 695 Park Avenue, New York, New York 10021, USA, US aut NATIONALLICENCE 700 1- Chatakondu Saikrishna Department of Biological Sciences, Hunter College of The City University of New York, 695 Park Avenue, New York, New York 10021, USA, US aut NATIONALLICENCE 700 1- Urbanski Diane Department of Biological Sciences, Hunter College of The City University of New York, 695 Park Avenue, New York, New York 10021, USA, US aut NATIONALLICENCE 700 1- Lopes Rui Department of Biological Sciences, Hunter College of The City University of New York, 695 Park Avenue, New York, New York 10021, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer