caa a22 4500 475746252 CHVBK 20180406123514.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s002849910028 doi (NATIONALLICENCE)springer-10.1007/s002849910028 Genetic Characterization of a New Thermotolerant Bacillus licheniformis Strain [Elektronische Daten] [Sonia A.L.V. Mendo, Isabel S. Henriques, António C.M. Correia, José M.C. Duarte] Abstract.: A potentially new thermotolerant B. licheniformis strain (code name I89), producer of an antibiotic active against Gram-positive bacteria, was genetically characterized and compared with the type strain B. licheniformis ATCC 10716, producer of bacitracin. Studies on DNA base composition (G + C content) and DNA reassociation revealed that the two strains show around 76% homology. Nevertheless, results obtained by rRNA hybridization, with a heterologous probe coding for most of the 16S region of the rRNA operon of Bacillus subtilis, revealed differences in the number of copies for that gene and in the hybridization pattern. Additionally, a different restriction digestion pattern was obtained when DNA was digested with the enzymes NotI, SmaI and analyzed by PFGE. The I89 strain holds a 7.6-kb plasmid not present in the reference strain. The existence of various unique restriction sites and also the stability of this plasmid make it ideal for the future development of a cloning and expression vector. Springer-Verlag New York Inc., 2000 Mendo Sonia A.L.V. Department of Biology, University of Aveiro, 3810 Aveiro, Portugal, PT aut Henriques Isabel S. Department of Biology, University of Aveiro, 3810 Aveiro, Portugal, PT aut Correia António C.M. Department of Biology, University of Aveiro, 3810 Aveiro, Portugal, PT aut Duarte José M.C. INETI, Azinhaga dos Lameiros, 1699 Lisboa Codex, Portugal, PT aut https://doi.org/10.1007/s002849910028 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002849910028 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mendo Sonia A.L.V. Department of Biology, University of Aveiro, 3810 Aveiro, Portugal, PT aut NATIONALLICENCE 700 1- Henriques Isabel S. Department of Biology, University of Aveiro, 3810 Aveiro, Portugal, PT aut NATIONALLICENCE 700 1- Correia António C.M. Department of Biology, University of Aveiro, 3810 Aveiro, Portugal, PT aut NATIONALLICENCE 700 1- Duarte José M.C. INETI, Azinhaga dos Lameiros, 1699 Lisboa Codex, Portugal, PT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer