caa a22 4500 475746317 CHVBK 20180406123514.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s002840010091 doi (NATIONALLICENCE)springer-10.1007/s002840010091 Mutations in the Bacillus subtilis Purine Repressor That Perturb PRPP Effector Function In Vitro and In Vivo [Elektronische Daten] [Manli Weng, Howard Zalkin] The Bacillus subtilis pur operon repressor (PurR) has a PRPP (5-phosphoribosyl 1-pyrophosphate) binding motif at residues 199-211. Two PurR PRPP binding region mutations (D203A and D204A) were constructed, and the effects on binding of repressor to the pur operon control site in vitro and on regulation of pur operon expression in vivo were investigated. PRPP significantly inhibited the binding of wild-type but not mutant PurR to pur operon control site DNA. In strains with the D203A and D204A mutations, pur operon expression in vivo was super-repressed by addition of adenine to the growth medium. These results support the role of PRPP in modulating the regulatory function of PurR in vivo. YabJ, the product of the distal gene in the bicistronic purR operon, is also required for PurR function in vivo. Springer-Verlag New York Inc., 2000 Weng Manli Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, P. R. China, CN aut Zalkin Howard Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA, US aut https://doi.org/10.1007/s002840010091 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010091 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Weng Manli Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, P. R. China, CN aut NATIONALLICENCE 700 1- Zalkin Howard Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer