caa a22 4500 475746376 CHVBK 20180406123514.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s002840010090 doi (NATIONALLICENCE)springer-10.1007/s002840010090 Genetic Fingerprinting of Brevibacterium linens by Pulsed-Field Gel Electrophoresis and Ribotyping [Elektronische Daten] [Paula T. Lima, António M. Correia] Members of Brevibacterium linens display physiological features that are relevant for cheese production. The genomes of five B. linens strains deposited on culture collections were compared by examining large restriction fragments on pulsed-field gel electrophoresis and detection of polymorphism at the level of 16S rRNA genes. Pulsed-field analysis with the endonucleases DraI and AsnI showed a characteristic restriction profile for each strain and allowed the calculation of genome sizes ranging between 3.2 and 3.9 Mbp. No linear genomic elements were detected. Polymorphisms at the level of 16S rRNA genes were revealed by hybridization with an oligonucleotide probe complementary to a universal domain of the 16S genes. An EcoRI fragment of 1.4 kb was identified as common to all strains under study. According to the number of positive bands detected by the probe, at least four rRNA operons must be present on the genome of the B. linens strains here studied. Springer-Verlag New York Inc., 2000 Lima Paula T. Centro de Biologia Celular, Departamento de Biologia, Universidade de Aveiro, 3810-193 Aveiro, Portugal, PT aut Correia António M. Centro de Biologia Celular, Departamento de Biologia, Universidade de Aveiro, 3810-193 Aveiro, Portugal, PT aut https://doi.org/10.1007/s002840010090 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010090 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lima Paula T. Centro de Biologia Celular, Departamento de Biologia, Universidade de Aveiro, 3810-193 Aveiro, Portugal, PT aut NATIONALLICENCE 700 1- Correia António M. Centro de Biologia Celular, Departamento de Biologia, Universidade de Aveiro, 3810-193 Aveiro, Portugal, PT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer