caa a22 4500 475746384 CHVBK 20180406123514.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s002840010093 doi (NATIONALLICENCE)springer-10.1007/s002840010093 Cloning of a New Bacillus thuringiensis cry1I -Type CrystalProtein Gene [Elektronische Daten] [Soo-Keun Choi, Byung-Sik Shin, Eun-Mee Kong, Hyune Mo Rho, Seung-Hwan Park] A new cry1I-type gene, cry1Id1, was cloned from a B. thuringiensis isolate, and its nucleotide sequence was determined. The deduced amino acid sequence of Cry1Id1 is 89.7%, 87.2%, and 83.4% identical to the Cry1Ia, Cry1Ib, and Cry1Ic proteins, respectively. The upstream sequence of the cry1Id1 structural gene was not functional as promoter in B. subtilis. The Cry1Id1 protein, purified from recombinant E. coli cells, had a toxicity comparable to that of Cry1Ia against Plutella xylostella, but it was significantly less active than Cry1Ia against Bombyx mori. Cry1Id1 was not active against the coleopteran insect, Agelastica coerulea. Springer-Verlag New York Inc., 2000 Choi Soo-Keun Laboratory of Microbial and Bioprocess Engineering, Korea Research Institute of Bioscience & Biotechnology (KRIBB), P.O. Box 115, Yusong, Taejon 305-600, Korea, KP aut Shin Byung-Sik Laboratory of Microbial and Bioprocess Engineering, Korea Research Institute of Bioscience & Biotechnology (KRIBB), P.O. Box 115, Yusong, Taejon 305-600, Korea, KP aut Kong Eun-Mee Laboratory of Microbial and Bioprocess Engineering, Korea Research Institute of Bioscience & Biotechnology (KRIBB), P.O. Box 115, Yusong, Taejon 305-600, Korea, KP aut Rho Hyune Mo Department of Molecular Biology and Research Center for Cell Differentiation, Seoul National University, Seoul, 151-742, Republic of Korea, KR aut Park Seung-Hwan Genome Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), P.O. Box 115, Yusong, Taejon 305-600, Republic of Korea, KR aut https://doi.org/10.1007/s002840010093 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010093 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Choi Soo-Keun Laboratory of Microbial and Bioprocess Engineering, Korea Research Institute of Bioscience & Biotechnology (KRIBB), P.O. Box 115, Yusong, Taejon 305-600, Korea, KP aut NATIONALLICENCE 700 1- Shin Byung-Sik Laboratory of Microbial and Bioprocess Engineering, Korea Research Institute of Bioscience & Biotechnology (KRIBB), P.O. Box 115, Yusong, Taejon 305-600, Korea, KP aut NATIONALLICENCE 700 1- Kong Eun-Mee Laboratory of Microbial and Bioprocess Engineering, Korea Research Institute of Bioscience & Biotechnology (KRIBB), P.O. Box 115, Yusong, Taejon 305-600, Korea, KP aut NATIONALLICENCE 700 1- Rho Hyune Mo Department of Molecular Biology and Research Center for Cell Differentiation, Seoul National University, Seoul, 151-742, Republic of Korea, KR aut NATIONALLICENCE 700 1- Park Seung-Hwan Genome Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), P.O. Box 115, Yusong, Taejon 305-600, Republic of Korea, KR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer