caa a22 4500 475746392 CHVBK 20180406123514.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s002840010084 doi (NATIONALLICENCE)springer-10.1007/s002840010084 Methanosarcina mazei Strain O1M9704, Methanogen with Novel Tubule Isolated from Estuarine Environment [Elektronische Daten] [Mei-Chin Lai, Chin-Ming Shu, Sheng-Chung Chen, Li-Jane Lai, Ming-Shing Chiou, Jack Jay Hua] Abstract.: A new methanogenic isolate, designated as strain O1M9704 (=OCM 667), was isolated from the sediment of the estuarine environment in Eriln Shi, Taiwan. This strain grew on trimethylamine and methanol, but it did not catabolize H2-CO2, acetate, or formate. Cells grew optimally at 37°C with 0.5% NaCl in neutral pH. The cells were stained Gram-negative, nonmotile, irregular coccus 0.3-0.6 μm in diameter. A comparison of 16S rDNA sequences phylogenetically related strain O1M9704 to Methanosarcina mazei. Gas vacuoles were observed both under phase contrast microscope and in thin sections in the electron microscope. Negative stain of electron micrographs showed a novel character of strain O1M9704 with tubule structure extended out of the cells. The tubule structure and gas vacuoles may benefit the adaptation of methanoarchaea in estuarine environment. Springer-Verlag New York Inc., 2000 Lai Mei-Chin Department of Botany, National Chung Hsing University, Taichung, 40227 Taiwan, R.O.C., TW aut Shu Chin-Ming Department of Botany, National Chung Hsing University, Taichung, 40227 Taiwan, R.O.C., TW aut Chen Sheng-Chung Department of Botany, National Chung Hsing University, Taichung, 40227 Taiwan, R.O.C., TW aut Lai Li-Jane Department of Botany, National Chung Hsing University, Taichung, 40227 Taiwan, R.O.C., TW aut Chiou Ming-Shing Food Industry Research & Development Institute, Hsinchu, Taiwan, R.O.C., TW aut Hua Jack Jay Food Industry Research & Development Institute, Hsinchu, Taiwan, R.O.C., TW aut https://doi.org/10.1007/s002840010084 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010084 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lai Mei-Chin Department of Botany, National Chung Hsing University, Taichung, 40227 Taiwan, R.O.C., TW aut NATIONALLICENCE 700 1- Shu Chin-Ming Department of Botany, National Chung Hsing University, Taichung, 40227 Taiwan, R.O.C., TW aut NATIONALLICENCE 700 1- Chen Sheng-Chung Department of Botany, National Chung Hsing University, Taichung, 40227 Taiwan, R.O.C., TW aut NATIONALLICENCE 700 1- Lai Li-Jane Department of Botany, National Chung Hsing University, Taichung, 40227 Taiwan, R.O.C., TW aut NATIONALLICENCE 700 1- Chiou Ming-Shing Food Industry Research & Development Institute, Hsinchu, Taiwan, R.O.C., TW aut NATIONALLICENCE 700 1- Hua Jack Jay Food Industry Research & Development Institute, Hsinchu, Taiwan, R.O.C., TW aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer