caa a22 4500 475746414 CHVBK 20180406123514.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s002840010086 doi (NATIONALLICENCE)springer-10.1007/s002840010086 Characterization of the Cryptic Plasmid pSBO2 Isolated from Streptococcus bovis JB1 and Construction of a New Shuttle Vector [Elektronische Daten] [Mutsumi Nakamura, Koretsugu Ogata, Takafumi Nagamine, Kiyoshi Tajima, Hiroki Matsui, Yoshimi Benno] A cryptic plasmid designated pSBO2 (3582 bp) was isolated from Streptococcus bovis JB1. The pSBO2 contained putative sites for a double-strand origin (dso), a small transcriptional repressor protein (Cop), countertranscribed RNAs (ctRNAs), and a replication protein (Rep), which were similar to those from pMV158 and pLS1, which were isolated from S. agalactiae, and pWVO1, isolated from Lactococcus lactis. The putative single-strand origin (sso) of pSBO2 was similar to pER341 and pST1, which were isolated from S. thermophilus. Recombinant plasmid designated pSBE2 was constructed to bind pECM184 vector and the DNA fragment containing sso, dso, Cop, ctRNAs, and Rep of pSBO2. When pSBE2 was introduced into S. bovis 12-U-1 and no8, the plasmids in the transformants had deleted the 160-bp fragment between sso and dso. This plasmid, designated pSBE2A, was capable of transforming Escherichia coli and S. bovis strains 12-U-1 and no8 on high frequency; therefore, pSBE2A is an effective shuttle vector. Springer-Verlag New York Inc., 2000 Nakamura Mutsumi Rumen Microbiology Research Team, STAFF-Institute, 446-1 Ippaizuka, Kamiyokoba Tsukuba, Ibaraki 305-0854, Japan, JP aut Ogata Koretsugu Rumen Microbiology Research Team, STAFF-Institute, 446-1 Ippaizuka, Kamiyokoba Tsukuba, Ibaraki 305-0854, Japan, JP aut Nagamine Takafumi Rumen Microbiology Research Team, STAFF-Institute, 446-1 Ippaizuka, Kamiyokoba Tsukuba, Ibaraki 305-0854, Japan, JP aut Tajima Kiyoshi Rumen Microbiology Research Team, STAFF-Institute, 446-1 Ippaizuka, Kamiyokoba Tsukuba, Ibaraki 305-0854, Japan, JP aut Matsui Hiroki Rumen Microbiology Research Team, STAFF-Institute, 446-1 Ippaizuka, Kamiyokoba Tsukuba, Ibaraki 305-0854, Japan, JP aut Benno Yoshimi Rumen Microbiology Research Team, STAFF-Institute, 446-1 Ippaizuka, Kamiyokoba Tsukuba, Ibaraki 305-0854, Japan, JP aut https://doi.org/10.1007/s002840010086 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010086 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakamura Mutsumi Rumen Microbiology Research Team, STAFF-Institute, 446-1 Ippaizuka, Kamiyokoba Tsukuba, Ibaraki 305-0854, Japan, JP aut NATIONALLICENCE 700 1- Ogata Koretsugu Rumen Microbiology Research Team, STAFF-Institute, 446-1 Ippaizuka, Kamiyokoba Tsukuba, Ibaraki 305-0854, Japan, JP aut NATIONALLICENCE 700 1- Nagamine Takafumi Rumen Microbiology Research Team, STAFF-Institute, 446-1 Ippaizuka, Kamiyokoba Tsukuba, Ibaraki 305-0854, Japan, JP aut NATIONALLICENCE 700 1- Tajima Kiyoshi Rumen Microbiology Research Team, STAFF-Institute, 446-1 Ippaizuka, Kamiyokoba Tsukuba, Ibaraki 305-0854, Japan, JP aut NATIONALLICENCE 700 1- Matsui Hiroki Rumen Microbiology Research Team, STAFF-Institute, 446-1 Ippaizuka, Kamiyokoba Tsukuba, Ibaraki 305-0854, Japan, JP aut NATIONALLICENCE 700 1- Benno Yoshimi Rumen Microbiology Research Team, STAFF-Institute, 446-1 Ippaizuka, Kamiyokoba Tsukuba, Ibaraki 305-0854, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer