caa a22 4500 475746422 CHVBK 20180406123514.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s002840010088 doi (NATIONALLICENCE)springer-10.1007/s002840010088 Mutations Permitting fdhF (Formate Dehydrogenase H) Expression in a selD Mutant of Salmonella typhimurium [Elektronische Daten] [Isabelle Chénier, Francine Dubé, Patrick C. Hallenbeck] Translation of fdhF mRNA, encoding formate dehydrogenase H, requires the context-specific insertion of selenocysteine at an opal codon. We have cloned the Tn10 insertion previously shown to block fdhF transcription in Salmonella typhimurium and shown that it lies in selD, which encodes phosphoselenate synthetase. A spontaneous mutant of the selD::Tn10 strain that expresses an fdhF::lacZ protein fusion (where lacZ is fused after the opal codon) was isolated and characterized. Suppression showed the same context requirement as previously reported for SelB insertion of selenocysteine. The suppressing mutation was mapped by P22 co-transduction to the 74-75 min of the Salmonella typhimurium genome. Springer-Verlag New York Inc., 2000 Chénier Isabelle Département de micribiologie et immunologie, Université de Montréal, C.P. 6128, Succursale Centre-ville, Montréal, Québec, H3C 3J7, Canada, CA aut Dubé Francine Département de micribiologie et immunologie, Université de Montréal, C.P. 6128, Succursale Centre-ville, Montréal, Québec, H3C 3J7, Canada, CA aut Hallenbeck Patrick C. Département de micribiologie et immunologie, Université de Montréal, C.P. 6128, Succursale Centre-ville, Montréal, Québec, H3C 3J7, Canada, CA aut https://doi.org/10.1007/s002840010088 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010088 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chénier Isabelle Département de micribiologie et immunologie, Université de Montréal, C.P. 6128, Succursale Centre-ville, Montréal, Québec, H3C 3J7, Canada, CA aut NATIONALLICENCE 700 1- Dubé Francine Département de micribiologie et immunologie, Université de Montréal, C.P. 6128, Succursale Centre-ville, Montréal, Québec, H3C 3J7, Canada, CA aut NATIONALLICENCE 700 1- Hallenbeck Patrick C. Département de micribiologie et immunologie, Université de Montréal, C.P. 6128, Succursale Centre-ville, Montréal, Québec, H3C 3J7, Canada, CA aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer