caa a22 4500 475746503 CHVBK 20180406123515.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s002840010157 doi (NATIONALLICENCE)springer-10.1007/s002840010157 Characterization of INTA 51-3, a New Atypical Strain of Bacillus thuringiensis from Argentina [Elektronische Daten] [Graciela B. Benintende, Joel E. López-Meza, Jorge G. Cozzi, Carlos F. Piccinetti, Jorge E. Ibarra] Several isolates of Bacillus thuringiensis native to Argentina obtained in a nationwide screening program showed atypical crystal morphology. One of these strains, INTA 51-3, was further characterized in order to determine other features like protein composition of its parasporal crystal, plasmid pattern, identification of cry genes and toxicological properties. B. thuringiensis INTA 51-3 (serovar tohokuensis) had an amorphous inclusion containing a major protein component of ca. 130 kDa. After trypsin digestion of solubilized crystals, SDS-PAGE resolved a unique protease-resistant peptide of ca. 90 kDa. The plasmid pattern from INTA 51-3 resembled that of the standard strain HD-1. However, Southern analysis showed no hybridization to fragments of cry1Aa, cry2Aa, cry3A, and cry11A genes. Degenerate primers were used for identification of the cry1 genes by PCR. Nevertheless, the presence of cry1 type gene(s) in B. thuringiensis INTA 51-3 was confirmed. Highly concentrated crystal suspensions showed to be weakly toxic only to lepidopteran species. Springer-Verlag New York Inc., 2000 Benintende Graciela B. Instituto de Microbiología y Zoología Agrícola, Instituto Nacional de Tecnología Agropecuaria, CC 25, (1712) Castelar, Argentina, Argentina aut López-Meza Joel E. Centro Multidisciplinario de Estudios en Biotecnología, Univ. Michoacana de San Nicolás de Hidalgo, Km 9.5 Carretera Morelia-Zinapécuaro, Morelia, Michoacán, México,, México aut Cozzi Jorge G. Instituto de Microbiología y Zoología Agrícola, Instituto Nacional de Tecnología Agropecuaria, CC 25, (1712) Castelar, Argentina, Argentina aut Piccinetti Carlos F. Instituto de Microbiología y Zoología Agrícola, Instituto Nacional de Tecnología Agropecuaria, CC 25, (1712) Castelar, Argentina, Argentina aut Ibarra Jorge E. Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del I.P.N., Apdo. Postal 629, 36500 Irapuato, Gto., M|$$|Aaexico, México aut Current Microbiology Springer-Verlag 41/6(2000-12-01), 396-401 0343-8651 41:6<396 2000 41 284 https://doi.org/10.1007/s002840010157 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010157 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Benintende Graciela B. Instituto de Microbiología y Zoología Agrícola, Instituto Nacional de Tecnología Agropecuaria, CC 25, (1712) Castelar, Argentina, Argentina aut NATIONALLICENCE 700 1- López-Meza Joel E. Centro Multidisciplinario de Estudios en Biotecnología, Univ. Michoacana de San Nicolás de Hidalgo, Km 9.5 Carretera Morelia-Zinapécuaro, Morelia, Michoacán, México,, México aut NATIONALLICENCE 700 1- Cozzi Jorge G. Instituto de Microbiología y Zoología Agrícola, Instituto Nacional de Tecnología Agropecuaria, CC 25, (1712) Castelar, Argentina, Argentina aut NATIONALLICENCE 700 1- Piccinetti Carlos F. Instituto de Microbiología y Zoología Agrícola, Instituto Nacional de Tecnología Agropecuaria, CC 25, (1712) Castelar, Argentina, Argentina aut NATIONALLICENCE 700 1- Ibarra Jorge E. Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del I.P.N., Apdo. Postal 629, 36500 Irapuato, Gto., M|$$|Aaexico, México aut NATIONALLICENCE 773 0- Current Microbiology Springer-Verlag 41/6(2000-12-01), 396-401 0343-8651 41:6<396 2000 41 284 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer