caa a22 4500 475746546 CHVBK 20180406123515.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s002840010161 doi (NATIONALLICENCE)springer-10.1007/s002840010161 Molecular Cloning and DNA Analysis of the Orotidine-5′-Phosphate Decarboxylase Gene from the Yeast Saccharomyces exiguus Yp74L-3 [Elektronische Daten] [Taisuke Hisatomi, Takuya Kodama, Hiroshi Toba, Masanao Moto-oka, Akihiro Watanabe, Michio Tsuboi] The orotidine-5′-phosphate decarboxylase gene of Saccharomyces exiguus Yp74L-3 was cloned as a DNA fragment complementing a ura4 mutation of this yeast. The coding region of the gene is 807 bp in length, and represents 68.7% similarity to the corresponding gene of S. cerevisiae (URA3). The cloned URA4 gene was shown to be located on the 790-kbp Chromosome (chr) VIII of S. exiguus Yp74L-3. The neighbor-joining phylogenetic tree based on the orotidine-5′-phosphate decarboxylase coding sequences indicates that S. exiguus Yp74L-3 is closely related to Kluyveromyces yeasts, as well as to a S. cerevisiae laboratory strain. Springer-Verlag New York Inc., 2000 Hisatomi Taisuke Department of Biotechnology, Faculty of Engineering, Fukuyama University, Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan, Japan aut Kodama Takuya Department of Biotechnology, Faculty of Engineering, Fukuyama University, Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan, Japan aut Toba Hiroshi Department of Biotechnology, Faculty of Engineering, Fukuyama University, Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan, Japan aut Moto-oka Masanao Department of Biotechnology, Faculty of Engineering, Fukuyama University, Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan, Japan aut Watanabe Akihiro Department of Biotechnology, Faculty of Engineering, Fukuyama University, Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan, Japan aut Tsuboi Michio Department of Biotechnology, Faculty of Engineering, Fukuyama University, Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan, Japan aut Current Microbiology Springer-Verlag 41/6(2000-12-01), 421-424 0343-8651 41:6<421 2000 41 284 https://doi.org/10.1007/s002840010161 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010161 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hisatomi Taisuke Department of Biotechnology, Faculty of Engineering, Fukuyama University, Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan, Japan aut NATIONALLICENCE 700 1- Kodama Takuya Department of Biotechnology, Faculty of Engineering, Fukuyama University, Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan, Japan aut NATIONALLICENCE 700 1- Toba Hiroshi Department of Biotechnology, Faculty of Engineering, Fukuyama University, Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan, Japan aut NATIONALLICENCE 700 1- Moto-oka Masanao Department of Biotechnology, Faculty of Engineering, Fukuyama University, Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan, Japan aut NATIONALLICENCE 700 1- Watanabe Akihiro Department of Biotechnology, Faculty of Engineering, Fukuyama University, Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan, Japan aut NATIONALLICENCE 700 1- Tsuboi Michio Department of Biotechnology, Faculty of Engineering, Fukuyama University, Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan, Japan aut NATIONALLICENCE 773 0- Current Microbiology Springer-Verlag 41/6(2000-12-01), 421-424 0343-8651 41:6<421 2000 41 284 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer