caa a22 4500 475746678 CHVBK 20180406123515.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s002840010123 doi (NATIONALLICENCE)springer-10.1007/s002840010123 Nucleotide Sequence of α-Galactosidase MEL Gene from Zygosaccharomyces mrakii [Elektronische Daten] [Yuji Oda, Tomoko Fujisawa] The region encompassing the α-galactosidase MELr gene was amplified from Zygosaccharomyces mrakii IFO 1835T by inverse-PCR and then sequenced. The nucleotide sequence of this region revealed a single open reading frame of 1410 bp encoding a 470 amino acid protein with a molecular weight of 51,909. The similarity of the deduced mature protein to other yeast α-galactosidases was 63.3% to Zygosaccharomyces cidri, 71.5% to Torulaspora delbrueckii, and 70.7-73.9% to Saccharomyces species. The nucleotide and amino acid sequences are deposited in the DDBJ/EMBL/GenBank database under Accession Number AB030209. Springer-Verlag New York Inc., 2000 Oda Yuji Upland Agriculture Research Center, Hokkaido National Agricultural Experiment Station, Memuro, Kasai, Hokkaido, 082-0071, Japan, JP aut Fujisawa Tomoko Department of Applied Biology, Kyoto Institute of Technology, Sakyo-ku, Kyoto 606-8585, Japan, JP aut https://doi.org/10.1007/s002840010123 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010123 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oda Yuji Upland Agriculture Research Center, Hokkaido National Agricultural Experiment Station, Memuro, Kasai, Hokkaido, 082-0071, Japan, JP aut NATIONALLICENCE 700 1- Fujisawa Tomoko Department of Applied Biology, Kyoto Institute of Technology, Sakyo-ku, Kyoto 606-8585, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer