caa a22 4500 475746708 CHVBK 20180406123515.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s002840010110 doi (NATIONALLICENCE)springer-10.1007/s002840010110 Formation and Stability of the Bacteriophage λ Replication Complexes in UV-Irradiated Escherichia coli [Elektronische Daten] [Alicja Wegrzyn, Grzegorz Wegrzyn] Bacteriophage λ replication complex, containing the phage-encoded O initiator protein protected from proteases by other elements of this complex, is a stable structure that can be inherited by one of the two daughter λ DNA copies after a replication round in Escherichia coli. In normal growth conditions in bacteria bearing a plasmid derived from bacteriophage λ, such a complex may be stable for many cell generations. However, it was found that this stable structure is disassembled under certain conditions, namely, after heat shock. Therefore, we asked whether other environmental stresses may cause disassembly of the λ replication complex. We found that UV irradiation of the host cells prevented formation of the stable λ replication complex (though not preventing phage replication), while the same UV doses did not affect the stability of the replication complex assembled prior to the irradiation. These results indicate that the stable λ replication complex, although sensitive to heat shock, is resistant to some other environmental stresses and that formation of at least two types of λ replication complexes is possible. Both stable and unstable λ replication complexes are functional because replication of λ DNA under conditions preventing formation of the stable complex proceeds efficiently. Springer-Verlag New York Inc., 2000 Wegrzyn Alicja Laboratory of Molecular Biology (affiliated with the University of Gdańsk), Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Kładki 24, 80-822 Gdańsk, Poland aut Wegrzyn Grzegorz Department of Molecular Biology, University of Gdańsk, Kładki 24, 80-822 Gdańsk, Poland aut https://doi.org/10.1007/s002840010110 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010110 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wegrzyn Alicja Laboratory of Molecular Biology (affiliated with the University of Gdańsk), Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Kładki 24, 80-822 Gdańsk, Poland aut NATIONALLICENCE 700 1- Wegrzyn Grzegorz Department of Molecular Biology, University of Gdańsk, Kładki 24, 80-822 Gdańsk, Poland aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer