caa a22 4500 475746740 CHVBK 20180406123515.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s002840010111 doi (NATIONALLICENCE)springer-10.1007/s002840010111 Current Status of Helicobacter Contamination of Laboratory Mice, Rats, Gerbils, and House Musk Shrews in Japan [Elektronische Daten] [Kazuo Goto, Hiroaki Ohashi, Akira Takakura, Toshio Itoh] The current status of Helicobacter contamination of laboratory mice, rats, gerbils, and house musk shrews from 47, 10, 4, and 3 colonies, respectively, in Japan was studied. Helicobacter was detected by reverse transcription (RT)-nested polymerase chain reaction (PCR) with Helicobacter genus-specific primers by using feces obtained from the animals. H. hepaticus, H. muridarum, H. bilis, H. rodentium, "Flexispira rappini”, and "H. suncus” were identified with species-specific primers. Other species of Helicobacter were identified by sequencing of PCR products amplified with genus-specific primers. For mice, H. hepaticus, H. bilis, H. rodentium including H. rodentium-like organism, "H. typhlonicus”-like organism, and H. westmeadii-like organism were detected from 12 (25.5%), 1 (2.1%), 18 (38.3%), 1 (2.1%), and 1 (2.1%) colonies, respectively. Forty-seven (5.7%) mice from six (12.8%) colonies were contaminated with both H. hepaticus and H. rodentium. H. rodentium was detected in rats from three (30.0%) colonies. In gerbils, H. hepaticus was detected in three (75.0%) colonies, but other species of Helicobacter were not detected. In house musk shrews, "H. suncus” was detected in animals from two (66.7%) colonies. Visible lesions in the liver, which showed multiple pale to white foci, were observed in 6/42, 5/174, and 1/21 mice infected with H. hepaticus, H. rodentium, and H. hepaticus/H. rodentium, respectively, and 1/14 gerbils infected with H. hepaticus. The results suggest the prevalence of these species of Helicobacter in mice, rats, gerbils, and house musk shrews in Japan. Springer-Verlag New York Inc., 2000 Goto Kazuo Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasakishi, Kanagawa, 216-0001, Japan, JP aut Ohashi Hiroaki Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasakishi, Kanagawa, 216-0001, Japan, JP aut Takakura Akira Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasakishi, Kanagawa, 216-0001, Japan, JP aut Itoh Toshio Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasakishi, Kanagawa, 216-0001, Japan, JP aut https://doi.org/10.1007/s002840010111 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010111 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Goto Kazuo Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasakishi, Kanagawa, 216-0001, Japan, JP aut NATIONALLICENCE 700 1- Ohashi Hiroaki Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasakishi, Kanagawa, 216-0001, Japan, JP aut NATIONALLICENCE 700 1- Takakura Akira Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasakishi, Kanagawa, 216-0001, Japan, JP aut NATIONALLICENCE 700 1- Itoh Toshio Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasakishi, Kanagawa, 216-0001, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer