caa a22 4500 475746759 CHVBK 20180406123515.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s002840010130 doi (NATIONALLICENCE)springer-10.1007/s002840010130 pS86, A New Theta-Replicating Plasmid from Enterococcus faecalis [Elektronische Daten] [Manuel Martínez-Bueno, Eva Valdivia, Antonio Gálvez, Mercedes Maqueda] The complete nucleotide sequence of the small (5149 bp) and cryptic plasmid pS86 from Enterococcus faecalis ssp. faecalis S-86 has been determined. Sequence analysis revealed six putative open reading frames (ORFs) encoding polypeptides of 28.3, 11.5, 8.4, 65.1, 7.3, and 11.96 kDa each. Based on sequence similarity, two cassettes have been identified in pS86: ORF1 codes for the replication initiation protein (Rep); ORF4 codes for a putative mobilization protein that shows similarities to Mob/Pre proteins from plasmids of Gram-positive bacteria. No function could be assigned to the other putative ORFs found. According to our results, pS86 plasmid could use a theta-mode of replication, similar to the recently described theta-type replicons from pUCL287 (Tetragenococcus halophila) and pLA1 or pLA105 (Lactobacillus acidophilus) plasmids. Springer-Verlag New York Inc., 2000 Martínez-Bueno Manuel Dpto. Microbiología, Facultad Ciencias, Univ. Granada c/Fuentenueva s/n, 18071-Granada, Spain, ES aut Valdivia Eva Dpto. Microbiología, Facultad Ciencias, Univ. Granada c/Fuentenueva s/n, 18071-Granada, Spain, ES aut Gálvez Antonio Dpto. Microbiología, Facultad C. Experimentales, Univ. Jaén, Spain, ES aut Maqueda Mercedes Instituto de Biotecnología, Univ. Granada, Spain, ES aut https://doi.org/10.1007/s002840010130 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010130 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martínez-Bueno Manuel Dpto. Microbiología, Facultad Ciencias, Univ. Granada c/Fuentenueva s/n, 18071-Granada, Spain, ES aut NATIONALLICENCE 700 1- Valdivia Eva Dpto. Microbiología, Facultad Ciencias, Univ. Granada c/Fuentenueva s/n, 18071-Granada, Spain, ES aut NATIONALLICENCE 700 1- Gálvez Antonio Dpto. Microbiología, Facultad C. Experimentales, Univ. Jaén, Spain, ES aut NATIONALLICENCE 700 1- Maqueda Mercedes Instituto de Biotecnología, Univ. Granada, Spain, ES aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer