caa a22 4500 475746767 CHVBK 20180406123515.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s002840010135 doi (NATIONALLICENCE)springer-10.1007/s002840010135 Targeted Disruption of sti 35, a Stress-Responsive Gene in Phytopathogenic Fungus Fusarium oxysporum [Elektronische Daten] [Pornthap Thanonkeo, Kouichi Akiyama, Sona Jain, Renkichi Takata] sti35 is one of the heat-shock genes in Fusarium oxysporum, which is a fungal pathogen for wilt disease in plants. We have isolated a genomic clone of sti35 and used it to create disruption mutations. Disruption of the sti35 coding region resulted in the loss of a 32-kDa protein present in heat-shocked cells. The disruption had no detectable effect on growth and development at various temperatures, nor on the ability to acquire thermotolerance in nutrient medium. But the sti35 disruptants showed increased thermotolerance, relative to the wild-type strain, when incubated in minimal medium after heat treatment. Springer-Verlag New York Inc., 2000 Thanonkeo Pornthap Faculty of Agriculture, Ehime University, Matsuyama 790-8566, Japan, JP aut Akiyama Kouichi Center for Gene Research, Ehime University, Matsuyama, Japan, JP aut Jain Sona Faculty of Agriculture, Ehime University, Matsuyama 790-8566, Japan, JP aut Takata Renkichi Faculty of Agriculture, Ehime University, Matsuyama 790-8566, Japan, JP aut https://doi.org/10.1007/s002840010135 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010135 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thanonkeo Pornthap Faculty of Agriculture, Ehime University, Matsuyama 790-8566, Japan, JP aut NATIONALLICENCE 700 1- Akiyama Kouichi Center for Gene Research, Ehime University, Matsuyama, Japan, JP aut NATIONALLICENCE 700 1- Jain Sona Faculty of Agriculture, Ehime University, Matsuyama 790-8566, Japan, JP aut NATIONALLICENCE 700 1- Takata Renkichi Faculty of Agriculture, Ehime University, Matsuyama 790-8566, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer