caa a22 4500 475746805 CHVBK 20180406123515.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s0028432708 doi (NATIONALLICENCE)springer-10.1007/s0028432708 Correlation of the Insecticidal Activity of the Bacillus thuringiensis A4 Strain Against Bactrocera oleae (Diptera) with the 140-kDa Crystal Polypeptide [Elektronische Daten] [Afroditi Sivropoulou, Lidia Haritidou, Eleni Vasara, Sofia Aptosoglou, Stefanos Koliais] The crystals of the soil-isolated Bacillus thuringiensis (Bt) strain A4 consist of two polypeptides with molecular mass of 140 kDa and 32 kDa that exhibit insecticidal activity against adult flies of Bactrocera oleae (Diptera). Plasmid curing applied to this strain resulted in the isolation of several subclones exhibiting alterations in their crystal polypeptides as well as two acrystalliferous subclones. The crystals of subclone 1.1 lacked the 32-kDa polypeptide and consisted uniquely of a 140-kDa polypeptide antigenically related to the parental 140-kDa crystal polypeptide. Additionally, the crystals of this subclone exhibited insecticidal activity against B. oleae equivalent to that of the parental strain. Therefore, the 32-kDa crystal polypeptide is dispensable for insecticidal activity, which appears to be dependent on the presence of the 140-kDa crystal polypeptide. Springer-Verlag New York Inc., 2000 Sivropoulou Afroditi Laboratory of General Microbiology, Section of Genetics, Development and Molecular Biology, School of Biology, Aristotle University, Thessaloniki 54006, Greece, GR aut Haritidou Lidia Laboratory of General Microbiology, Section of Genetics, Development and Molecular Biology, School of Biology, Aristotle University, Thessaloniki 54006, Greece, GR aut Vasara Eleni Laboratory of General Microbiology, Section of Genetics, Development and Molecular Biology, School of Biology, Aristotle University, Thessaloniki 54006, Greece, GR aut Aptosoglou Sofia Laboratory of General Microbiology, Section of Genetics, Development and Molecular Biology, School of Biology, Aristotle University, Thessaloniki 54006, Greece, GR aut Koliais Stefanos Laboratory of General Microbiology, Section of Genetics, Development and Molecular Biology, School of Biology, Aristotle University, Thessaloniki 54006, Greece, GR aut https://doi.org/10.1007/s0028432708 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s0028432708 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sivropoulou Afroditi Laboratory of General Microbiology, Section of Genetics, Development and Molecular Biology, School of Biology, Aristotle University, Thessaloniki 54006, Greece, GR aut NATIONALLICENCE 700 1- Haritidou Lidia Laboratory of General Microbiology, Section of Genetics, Development and Molecular Biology, School of Biology, Aristotle University, Thessaloniki 54006, Greece, GR aut NATIONALLICENCE 700 1- Vasara Eleni Laboratory of General Microbiology, Section of Genetics, Development and Molecular Biology, School of Biology, Aristotle University, Thessaloniki 54006, Greece, GR aut NATIONALLICENCE 700 1- Aptosoglou Sofia Laboratory of General Microbiology, Section of Genetics, Development and Molecular Biology, School of Biology, Aristotle University, Thessaloniki 54006, Greece, GR aut NATIONALLICENCE 700 1- Koliais Stefanos Laboratory of General Microbiology, Section of Genetics, Development and Molecular Biology, School of Biology, Aristotle University, Thessaloniki 54006, Greece, GR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer