caa a22 4500 475746813 CHVBK 20180406123515.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s002840010128 doi (NATIONALLICENCE)springer-10.1007/s002840010128 Enzyme Activity of Lectins from the Nitrogen-Fixing Soil Bacterium Bacillus polymyxa [Elektronische Daten] [Ulyana Yu. Mel'nikova, Lidiya V. Karpunina, Nadezhda V. Ostakhina, Vladimir V. Ignatov] Lectins LI and LII, localized on the surface of the nitrogen-fixing soil bacterium Bacillus polymyxa 1460, were shown to possess proteolytic activity. A relationship was found between the proteolytic and hemagglutinating activities of the lectins. Blocking of hemagglutinating activity with specific carbohydrate haptens led to significant changes in the enzyme activity of both lectins. When lectin activity was blocked with glucuronic acid and fructose-1,6-diphosphate, the proteolytic activity of both LI and LII declined, whereas incubation with d-galactosamine and d-glucosamine promoted increases in the proteolytic activity of LII. This study proposes that the molecules of the B. polymyxa lectins may have two centers on their surfaces: one responsible for lectin activity and the other for proteolytic activity. Springer-Verlag New York Inc., 2000 Mel'nikova Ulyana Yu Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Pr. Entuziastov, Saratov 410015, Russia, RU aut Karpunina Lidiya V. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Pr. Entuziastov, Saratov 410015, Russia, RU aut Ostakhina Nadezhda V. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Pr. Entuziastov, Saratov 410015, Russia, RU aut Ignatov Vladimir V. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Pr. Entuziastov, Saratov 410015, Russia, RU aut https://doi.org/10.1007/s002840010128 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010128 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mel'nikova Ulyana Yu Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Pr. Entuziastov, Saratov 410015, Russia, RU aut NATIONALLICENCE 700 1- Karpunina Lidiya V. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Pr. Entuziastov, Saratov 410015, Russia, RU aut NATIONALLICENCE 700 1- Ostakhina Nadezhda V. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Pr. Entuziastov, Saratov 410015, Russia, RU aut NATIONALLICENCE 700 1- Ignatov Vladimir V. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Pr. Entuziastov, Saratov 410015, Russia, RU aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer