caa a22 4500 475746821 CHVBK 20180406123515.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s002840010137 doi (NATIONALLICENCE)springer-10.1007/s002840010137 Overexpression of Protein Disulfide Isomerase in Aspergillus [Elektronische Daten] [Hala El-Adawi, Nguyen Q. Khanh, Hans Gassen] One of the major problems with the production of biotechnologically valuable proteins has been the purification of the product. For Escherichia coli and Saccharomyces cerevisiae, there are several techniques for the purification of intracellular proteins, but these are time consuming and often result in poor yields. Purification can be considerably facilitated, if the product is secreted from the host cell. In the work presented, we have constructed an expression vector (pSGNH2) for the secretion of protein disulfide isomerase (PDI; EC 5.3.4.1) from Aspergillus niger, in which the retention signal His-Asp-Glu-Leu (H-D-E-L) was modified to Ala-Leu-Glu-Gln (A-L-E-Q) via the polymerase chain reaction (PCR) method. The PDI gene was placed under the control of the A. oryzaeα-amylase promoter. This expression vector was transformed into A. niger NRRL3, resulting in PDI secretion into the medium. The catalytic activity of overexpressed PDI from A. niger was indistinguishable from that of PDI isolated from bovine liver. With further strain improvement and optimization of culture conditions, it could be possible to raise the PDI production to the bioprocessing scale. Springer-Verlag New York Inc., 2000 El-Adawi Hala Institut für Biochemie, Petersen str. 22, Technische Universität, Darmstadt, D-64287, Darmstadt, Germany, DE aut Khanh Nguyen Q. Institut für Biochemie, Petersen str. 22, Technische Universität, Darmstadt, D-64287, Darmstadt, Germany, DE aut Gassen Hans Institut für Biochemie, Petersen str. 22, Technische Universität, Darmstadt, D-64287, Darmstadt, Germany, DE aut https://doi.org/10.1007/s002840010137 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010137 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- El-Adawi Hala Institut für Biochemie, Petersen str. 22, Technische Universität, Darmstadt, D-64287, Darmstadt, Germany, DE aut NATIONALLICENCE 700 1- Khanh Nguyen Q. Institut für Biochemie, Petersen str. 22, Technische Universität, Darmstadt, D-64287, Darmstadt, Germany, DE aut NATIONALLICENCE 700 1- Gassen Hans Institut für Biochemie, Petersen str. 22, Technische Universität, Darmstadt, D-64287, Darmstadt, Germany, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer