caa a22 4500 47574683X CHVBK 20180406123515.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s002840010125 doi (NATIONALLICENCE)springer-10.1007/s002840010125 Degradation of Glycinebetaine by Betaine-Homocysteine Methyltransferase in Aphanothece halophytica [Elektronische Daten] Effect of Salt Downshock and Starvation [Aran Incharoensakdi, Rungaroon Waditee] We have investigated conditions leading to the degradation of glycinebetaine in Aphanothece halophytica and have shown the activity of betaine-homocysteine methyltransferase (BHMT). The intracellular glycinebetaine level was decreased approximately 50% after 36 h salt downshock from 2.0 m NaCl medium to 0.5 m NaCl medium. A slight additional decrease of glycinebetaine occurred when salt downshock was combined with dark treatment. The omission of carbon and nitrogen sources in the growth medium further decreased intracellular glycinebetaine. The activity of BHMT increased from 0 to 460 nmol h−1mg−1 after 3 h salt downshock. Higher strength of salt downshock resulted in higher activity of the enzyme. Small increase of the enzyme activity was also observed when A. halophytica was deprived of carbon and nitrogen sources in the growth medium. Springer-Verlag New York Inc., 2000 Incharoensakdi Aran Laboratory of Biochemical Products, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand, TH aut Waditee Rungaroon Laboratory of Biochemical Products, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand, TH aut https://doi.org/10.1007/s002840010125 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010125 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Incharoensakdi Aran Laboratory of Biochemical Products, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand, TH aut NATIONALLICENCE 700 1- Waditee Rungaroon Laboratory of Biochemical Products, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand, TH aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer