caa a22 4500 475746880 CHVBK 20180406123516.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s002840010129 doi (NATIONALLICENCE)springer-10.1007/s002840010129 Kim Ho-San Lab. of Insect Pathology & Genetic Engineering, Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Seoul National University, Suwon 441-744, Korea, KP aut Comparative Study of the Frequency, Flagellar Serotype, Crystal Shape, Toxicity, and cry Gene Contents of Bacillus thuringiensis from Three Environments [Elektronische Daten] [Ho-San Kim] A number of Bacillus thuringiensis isolates from sericultural farm, soil, and granary samples in Korea were found. B. thuringiensis isolates were predominant in granary (40%), followed by sericultural farm (33% and 25% in the spring and fall isolation), and soil (10%). In toxicity tests for three areas, lepidopteran-active isolates were rich in the spring of sericultural farm and granary, but the fall isolation of sericultural farm displayed that a large number of B. thuringiensis isolates having dual specificity against both lepidopteran and dipteran larvae were found. The soil showed even distribution against lepidoptera and/or diptera. Most of B. thuringiensis isolates showed strong toxicity against tested insects. PCR analysis using cryI, cryII, cryIII, cryIV, and cryV gene-specific primers for determination of the cry gene contents of B. thuringiensis isolates indicated that the frequency of the cryIA, cryIC, cryID, and cryII among cry genes predominated, and the cryIB, cryIE, cryIF, cryIG, and cryIV were not popular. In contrast, no PCR products were detected for the cryIII and cryV templates. Several B. thuringiensis isolates produced unusual PCR products and complicated combinations consisting of multiple cry genes. Seven out of 11 B. thuringiensis isolates undetected by specific primers from sericultural farm, all out of 9 isolates from soil, and 19 out of 25 isolates from granary were toxic to lepidoptera and/or diptera. In addition, five nontoxic isolates of sericultural farm, all of five nontoxic isolates of soil, and 13 nontoxic isolates of granary produced the expected PCR products. PCR results showed varied distribution of cry genes for three areas, respectively. An evaluation of this novel activity demands that several criteria be measured: the frequency, flagellar serotype, crystal morphology, toxicity, and combination of the cry genes. Springer-Verlag New York Inc., 2000 https://doi.org/10.1007/s002840010129 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002840010129 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Kim Ho-San Lab. of Insect Pathology & Genetic Engineering, Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Seoul National University, Suwon 441-744, Korea, KP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer